Effect Of Bioaugmentation On Estrogenic Activity And Microbial Diversity During Wastewater Treatment

ObjectiveThis study through investigated estrogenic activity distribution and removal from an urban sewage treatment plant,to evaluate the effect of bioaugmentation on the remove estrogenic activity and microbial diversity during wastewater treatment.Method1.Bioaugmentation was used in series production lines I which was provided by the winning bidder of WWTP.The same batch of commercially available nitrifying bacteria was added to the aerobic stage of aeration tank periodically and quantitatively in accordance to the scheduled plan by key technicians of the bidding company,for 33 days,747 kg of bacteria in total.Conventional chemical experiment was used to test COD,NH₄⁺-N and TP concentration in the secondary effluent of I series production line of the WWTP during the application of bioaugmentation technique to evaluate its impact on the removal of conventional pollutants.2.SPE-GC-MS method was used to determine the content of 6 EEDs?E1,E2,EE2,BPA,4-NP and 4-OP?in the sewage from series production lines I.SPE-YTH method was used to determine the estradiol equivalents in the sewage from series production lines I,to analyze the effect of bioaugmentation on the removal of estrogenic activity.3.Illumina MeSequence platform was used to analyze microbial community diversity of activated sludge from series production lines I during the application of bioaugmentation.Result1.The COD and NH₄⁺-N removal rate of series production lines I with bioaugmentation technique was higher than that without this technique during the same treatment process of series production lines ??P<0.05?.2.6 EEDs determined by GC-MS method presented a good linear relationship and the correlation coefficient was higher than 0.999.Though experiments of average recoveries of standard and RSD,the results showed the method was accurate,sensitive.The E2-U dose-response curve determined by YTH was good,R2 was 0.99 and EC50 5.59×10-11mol/L.The results of GC-MS showed that E1,E2,EE2 and BPA?4-OP and 4-NP were not detected?were mainly distributed in water intake and primary sedimentation tank.E1 content was 27ng/L⁵32ng/L;E2 content was 78ng/L²050ng/L;EE2 content was 28.5ng/L¹900ng/L;BPA content was 22.8ng/L¹420ng/L.BPA?the other 5 were not detected?was the main EEDs in secondary sedimentation tank and its content was 22.8ng/L⁶11ng/L.The results of SPE-YTH showed that the EEQ in secondary sedimentation tank were lower than those of in water intake and primary sedimentation tank?P<0.05?.After the application of bioaugmentation,the removal rate of estrogenic activity increased from?76.38±1.62?% to?89.94±0.35?% in series production lines I?P<0.05?.4.The analysis result of Illumina MeSequence showed that microbial ? diversity index in the activated sludge increased,the taxa abundance of the main phylum actinobacteria,firmicutes and chloroflexi increased?P<0.05?.Conclusion1.This bioaugmentation could effectively improve decontamination capability on conventional pollutants and estrogenic activity of the sewage treatment plant.and this effect was probably related to the improvement of microbial community diversity in activated sludge.2.This bioaugmentation mainly improved the relative abundance of actionbacteria,firmicutes and Chloroflexi in activated sludge.