Study Of ?-glucosidase Immobilized Design And Synthesis Of Rare Ginsenosides

?-glucosidase(Tnap0602)derived from Thermotoga Naphthophila RUK-10 can efficiently transform ginsenoside Rb1 to form rare ginsenoside Rd.In this paper,a novel ?-glucosidase(Carbamate-binding module)The method of immobilization was used to study the immobilization method of heteroenzyme,which laid the foundation for the application of ?-glucosidase.In this paper,two engineered bacteria capable of expressing the fusion proteins0602-CBM2 and 0602-CBM3 were constructed and the target protein was purified.The purified protein was adsorbed to microcrystalline cellulose,regenerated amorphous cellulose and filter paper pulp,respectively.The preliminary immobilization of ?-glucosidase was achieved and the ginsenoside conversion experiment was carried out.The results showed that the maximum binding capacity of microcrystalline cellulose of recombinant enzyme 0602-CBM2 was 2mg / ml,while0602-CBM3 did not bind to microcrystalline cellulose,regenerated amorphous cellulose and filter paper pulp.The results showed that the immobilized ?-glucosidase could be used in the re-use of immobilized enzyme,and the activity of immobilized?-glucosidase could be improved after the repeated use of immobilized enzyme0602-CBM2 for three times,The HPLC assay showed that a single product peak was evident at 35 minutes compared to the extract without the enzyme.