The Research Of Nano Drug Delivery System Based On CdTe Quantum Dots Or CuS Nanoparticles

During the process of chemotherapy for cancer,the multidrug resistance can always reduce the concentration of anticancer drugs in cancer cells,leading to treatment failure and high recurrence of the tumor.Thus we used the inorganic nanomaterials(CdTe QDs and CuS nanoparticles)coloaded with chemotherapy drugs and sensitizers to reverse the multidrug resistance.1.Through the electrostatic interaction,cysteamine-modified cadmium tellurium quantum dots(Cys-CdTe QDs)was coloaded by anticancer drug(DNR)and chemotherapy sensitizer(GA).The electrostatic binding is so easy to break in the acidic environment so that a large number of drugs will release(cumulative release was up to 90%).Results of the western blotting tests showed that GA could enhance the sensitivity of drug-resistant cells to DNR by reducing the high expression of efflux pump(P-gp)and increase the ratio of apoptosis-associated protein Bax/Bcl-2.With the help of Cys-CdTe QDs and GA,DNR-GA-Cys-CdTe NPs could increase the accumulation of DNR in cancer cells,induce apoptosis of cancer cells,inhibit tumor growth and reverse multidrug resistance of tumor.Besides,the special optical properties of quantum dots made this drug-loading system useful for tracer and cell imaging.This idea of the multidrug delivery system would be a promising strategy to overcome MDR against the lymphoma.2.DNR and GA were coloaded to CuS nanoparticles by GFLG,which could be specifically digested with Cath B in the tumor.As a result,DNR was stable loaded on CuS nanoparticles in the normal blood circulation.However,when the drug delivery system reached the tumor tissue,the cumulative release of DNR would increase rapidly(>50%),thus enhancing the bioavailability of DNR and reducing the side effects on normal tissue.At the same time,we modified the anti-CD22 antibody on the surface of nanoparticles,which would provide the additional active targeting function for drug delivery system so that the concentration of DNR in the cancer cells will be large increased.CuS nanoparticles have a strong absorption in the near infrared region(1030 nm),which could be used for photothermal therapy.It could meet the requirements of photothermal therapy as the temperature of this material grew 23.7 ? within 15 minutes at the laser wavelength of 808 nm.The effects of antibody and the cell/animal experiments of CuS-GFLG-DNR/GA-anti CD22 remain to be further explored.