Construction And Study Of Novel Photochemistry Sensors For MicroRNA-21 And JEV

The detection of tumor markers and viruses is of great significance for disease diagnosis and treatment,and the development of simple and high sensitivity methods for tumor markers and virus detection is imperative.In this article,a fluorescent sensors was structured for highly sensitive detection of micro RNA-21 by utilizing the unique optical properties of 2-aminopurine?2-AP?.A highly selective resonance light sensor for Japanese encephalitis virus?JEV?detection was structured by combining the molecular imprinting technology with resonance light detection technology.The main research work is as follows:1.A highly sensitive and selective sensor for mi RNA detection was developed based on 2-AP probe and signal amplification technique.A low detection limit of 4 p M was obtained due to the protection provided by the G-quadruplex and catalyzed hairpin assembly?CHA?signal amplification.It is worth mentioning that the proposed strategy was free of quenchers and enzymes.In addition,the developed sensor can be used to detect overexpressed mi RNA-21 from human breast cancer cell lysate,indicating the proposed method exhibits potential application to the early diagnosis of various cancers.2.A quencher-free and enzyme-free sensor for highly sensitive mi RNA detection was developed based on 2-AP probe and signal amplification technique.The 2-AP probe needs no quenchers compared with conventional MBs due to the inherent property of 2-AP,which fluorescence in double-stranded DNA?ds DNA?is much lower than that in single-stranded DNA?ss DNA?.Furthermore,the proposed signal amplification technique is enzyme-free,making it more simple and cost effective.A wide linear range from 40 p M to 40 n M?R2 = 0.999?was achieved by this assay.Moreover,this sensor can be used to monitor mi RNA-21 expression levels in cancer cells,proving that the new proposed sensor has certain application value on biomedical research and early clinical diagnosis.3.A resonance light scattering?RLS?sensor for fast and highly selective detection of JEV was developed based on surface-imprinted polymers.To solve the problem about long adsorption time and high non-specific adsorption,the recognition platform was Fe₃O₄/Si O2 magnetic MIPs prepared by surface molecular imprinting technique.The target virus can be captured by the imprinted particles fast and selectively,resulting in increase of the RLS intensity.It is worth mentioning that the absorption equilibrium could be achieved within 20 min.Moreover,in selective adsorption experiment,imprinted nanoparticles exhibited a high specific recognition of the template virus over the non-imprinted nanoparticles and JEV could be selectively recognized by the imprinted particles from the other two viruses.Finally,the serum samples experiment proved that the proposed sensor has certain application value on the detection and isolation of virus.