Simultaneous Electrochemical Analysis Of Purine Compounds By Nano Clay Mineral Composite Electrodes

Gout is a kind of universal existence inflammatory disease with repetitive relapse,which have been appeared in the middle of the twentieth century in china.The study found that the direct cause of gout was the excessive uric acid(UA),while the root cause of gout was the increase of the purine content.Therefore,it is of great significance and application value to develop a rapid,simple and efficient method for the analysis of purine compounds.Compared with the traditional detection methods,electrochemical sensing has become an important research direction because of its good selectivity,high sensitivity,easy to operate,low detection cost and on-line analysis.This paper combined the advantages of nano-clay materials and carbon nanomaterials,and used the good hydrophilicity and adhesion of sodium carboxymethyl cellulose(CMC),has successfully prepared the waterprocessable,economic and environmental-friendly nano composite sensor for the simultaneous determination of purine compounds.The study was carried out with the characteristics of selectivity,sensitivity,stability and practicability of the sensor,and it was successfully applied to the detection of purine compounds in real samples.The specific conclusions were as follows:(1)By selecting the strong adsorption of halloysite nanotubes(HNTs)and with good conductivity and catalytic ability of carboxyl-functionalized multi-walled carbon nanotubes(fMWCNTs)as main material,and with the help of CMC aqueous solution with good adhesive property and hydrophilicity,the CMC-HNTs-f MWCNTs/GCE was successfully fabricated.The composite modified electrode has good stability,selectivity and sensitivity,and can be used for simultaneous electrochemical detection of UA,adenine(A)and guanine(G)under the optimum conditions,and their linear ranges were 1 – 130?mol L-1,0.05 – 9 ?mol L-1 and 0.3 – 25 ?mol L-1,respectively,and the detection limits were 0.5 mol L-1,0.018 ?mol L-1 and 0.019 ?mol L-1,respectively.In addition,the CMCATP-NG modified electrode was successfully applied to simultaneous determination of UA,XA and HX in fruit and vegetable samples.(2)The attapulgite(ATP)with strong adsorption and large specific surface area and the nitrogen doped graphene(NG)with good conductive property were dispersed in the aqueous solution of CMC,and the modified electrode CMC-ATP-NG/GCE was successfully prepared that can simultaneous detect UA,xanthine(XA)and hypoxanthine(HX).The modified electrode has good stability,selectivity and sensitivity,and the linear range of UA,XA and HX were 0.5 – 60 ?mol L-1,0.2 – 20 ?mol L-1 and 0.6 – 55 ?mol L-1,respectively,and their detection limits were 0.1 ?mol L-1,0.05 ?mol L-1 and 0.4 ?mol L-1,respectively.In addition,the CMC-ATP-NG modified electrode was successfully applied to simultaneous determination of UA,XA and HX in fruit and vegetable samples.