Effect And Its Mechanism Of Trans Fatty Acids On Migration Of Human Trophoblast Cells

Studies have shown that trans fatty acids produced during food processing may associated with many kinds of diseases.It was found that trans fatty acids could accumulate in the embryos and had negative correlation with the weight of new borns,but the effects of trans fatty acids on the placenta are still unknown.However,the migration of trophoblast cells influence the formation of placenta and it's the crucial part of the development of embryos.Therefore,in this study,we investigated the effect of elaidic acid and linoelaidic acid on the migration of human trophoblast cells and its related mechanism.Trophoblast tissues and trophoblast cells were cultured in vitro,and used culture-tissue migration method,scratch assay and Transwell assay to analyze the effect of elaidic acid and linoelaidic acid on the migration of trophoblast cells.Results have shown that elaidic acid and linoelaidic acid inhibited the migration of trophoblast cells and shown time-and dose-dependent manner.Furthermore,through Western Blot assay and gelatin zymography assay we found that elaidic acid and linoelaidic acid decreased the expression of ?-catenin and MMP-2.The results suggested that the effect of trans fatty acids on the migrate inhibition of trophoblast cells may related to Wnt/?-catenin pathway and the secretion of MMP-2.Using ?-catenin activator Li+ and combined with culture-tissue migration method,scratch assay and Transwell assay,we found that Li+ had antagonistic action on the migrate suppression of trophoblast cells by elaidic acid and linoelaidic acid.Furthermore,Li+ also had antagonistic action on the decrease of ?-catenin and MMP-2.In conclusion,elaidic acid and linoelaidic acid both have suppress effect on migration of human trophoblast cells and may involved to the decrease of ?-catenin and MMP-2.