Development Of Folate Graft Nanoparticles And Release Behavior As Drug Carriers

With polyethyleneimine(PEI)and ammonia propyl triethoxy silane(APTES)as the organic model,the porous CaCO3 was prepared through a simple step precipitation process,and then the prepared CaCO3 experimental conditions was optimized by the scheme of orthogonal experiment.The hollow hydroxyapatite(HAP)microspheres were successfully synthesized using hollow CaCO3 microspheres as template.Based on the carboxy group with folic acid and the amino of aminated hydroxyapatite(HAP-NH2)can occur amidation,folic acid was grafted onto the surface of APTES modified hydroxyapatite(HAP).The folic acid modified hydroxyapatite(HAP)particles(FA-APTES-HAP)can be used as a drug delivery carrier of doxorubicin(DOX),and the adsorption model and release model of doxorubicin(DOX)were also studied.Scanning electron microcopy images revealed that the hydroxyapatite(HAP)were approximately spherical with porous on the surface and the particle size is about 1-1.5um.The specific surface area test shows that the hydroxyapatite(HAP)specific surface is 34.58m2/g,and the micropore diameter is 17.89nm.The presence of biopolymers in the matrices was confirmed by Fourier transform infrared(FTIR)spectroscopies,hinting that the folic acid successfully grafted onto the surface of the hydroxyapatite(HAP).As for the drug loading reserch found that the adsorption equilibrium time is 12 hours and the adsorption model satisfies the Freundlich equation by measure the adsorption capacity in every time and every concentration.The release of doxorubicin(DOX)study shows that the drug release reached 83.58%in the 6.0 pH,and have 36 hours releasing time.And The Higuchi model and Riger-Peppas model can explain the release process.Due to the targeted function of the folic acid,that can reduce the damage on the normal cells in the drug delivery.So the particles of folic acid grafted onto the hydroxyapatite(HAP)suit as the carrier of anticancer drugs.