Study On The Cell Cycle Arrest Mediated By Cd-induced DNA Damage In Arabidopsis Seedlings

It is reported that the aberrant expression of cell cycle regulation and DNA repair genes can result in abnormal cell proliferation and genomic instability in eukaryotic cells under different stresses. In the thesis, Arabidopsis thaliana (Arabidopsis) seedlings were grown hydroponically on 0.5×M&S media containing cadmium (Cd) at 0-2.5 mg·L-1 for treatment of 5d. Realtime Q-PCR analysis revealed that expression of DNA damage repair and cell cycle regulation genes, including BRCA1, MRE11, WEE1, CDKA;1 and PCNA1, showed an inverted U-shaped dose-response, In contrast, notably reduced expression was observed for G1-to-S transition-ralated genes, Histone H4, E2Fa and PCNA2; DSB end processing, GR1,and DNA mismatch repair, MSH2, MSH6 and MLH1 genes in root tips exposed to 0.125-2.5 mg·L-1 Cd for 5 d. Flow cytometry (FCM) analysis revealed significant increases of cells with a 2C nuclear content and with a 4C and 8C nuclear content under Cd stresses of 0.125 and 1-2.5 mg·L-1 respectively. Our results suggest that 0.125 mg·L-1 Cd-induced DNA damage induced the marked Gl/S arrest, leading to accelerated growth in root tips, while 1.0-2.5 mg·L-1 Cd-induced DNA damage caused a notable G2/M arrest in root tips, leading to reduced growth in root tips. There may be a protective mechanism that prevents cells with damaged DNA from dividing under Cd stress.