| Sterigmatocystin, is the secondary metabolites which are produced by Aspergillus flavus,Bipolarisspp,Aspergillus nidulans and other fungi. It can mostly contaminate wheat,corn, peanuts, Soybean and other food crops and animal fodder.It is one of the most common mycotoxin pollutants and widely be found in nature. People and animal eat food which containing Sterigmatocystin will cause a wide range of toxic effects. Therefore, it is necessary to establish an efficient separation method for detection of Sterigmatocystin in foods.In our work, We synthesized 1-vinyl-3-amino imidazolium hydrobromide ionic liquid and 1-vinyl-3-butene imidazolium bromine ionic liquid as functional monomer and crosslinker in the imprinting process. In the paper, two types of molecularly imprinted polymers were prepared, which have high adsorptive selectivity to ST. In the experiment,1,8-dihydroxyanthraquinone be used as an alternative template, 1 -vinyl-3-amino imidazolium hydrobromide as functional monomer, 1-vinyl-3-butene imidazolium bromine as crosslinker, AIBN as initiator, molecularly imprinted Poly(Ionic Liquid)s polymers was prepared using a in-situ polymerization successfully. Under the optimal conditions, the prepared molecularly Poly(Ionic Liquid)polymers capillary monolithic column exhibited homogenous and porous skeleton structure with excellent permeability and good mechanical stability.The molecularly poly(ionic liquid)polymers capillary monolithic column coupled with CE method was established to detect ST residues in food. 1,8-dihydroxyanthraquinone used as an alternative template, 1-vinyl-3-amino imidazolium hydrobromide as functional monomer, 1-vinyl-3-butene imidazolium bromine as crosslinker, AIBN as initiator, molecularly imprinted polymer materialswas prepared using a bulk polymerization successfully. The molecularly poly(ionic liquid imprinted polymer) is characterized using infrared spectroscopy, scanning electron microscopy techniques. To verify the adsorption properties of molecularly poly(ionic liquid imprinted polymer, adsorption experimentswere processed, and the results showed that MIPs has a higher selection adsorption to ST. The method using molecularly poly(ionic liquid imprinted polymer) solid phase extraction associated with HPLC was established to detect the ST content in the sample.Molecularly imprinted Poly(Ionic Liquid)s polymers was prepared using a in-situ polymerization. The Sterigmatocystin and its structural analogue aflatoxin B1 were isolated by CE method. The separation of Sterigmatocystin and aflatoxin B1 was achieved by optimizing the separation conditions.The average recovery was between 80.4%-91.6% by molecularly poly(ionic liquid imprinted polymer) solid phase extraction associated with HPLC method of soybean, corn, rice and purification effect is good .In a consequence, it is proved that the analytical method is accurate and feasible. |
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