Detection Of Clostridium Botulium In Milk Powder

Clostridium botulinum is anaerobic Clostridium,gram positive bacteria(about 4×1 ?m),more than one in two parallel sides,blunt ends Park,straight or slightly curved rod-shaped,spore is located in the secondary side,larger than the cell width,the cells were oval,tennis racket shaped.There are 4 to 8 weeks of flagella,slow movement,no capsule.Clostridium botulinum can produce botulinum toxin(exotoxin),is a neurotoxin that can cause human death.Botulinum toxin is not heat,boiling water boiled lmin or 75 ? heating of 5?10min toxin can be completely destroyed,in the conditions of adaptation,the toxicity can be activated by trypsin.For food or detection in the environment of Clostridium botulinum are required for enrichment culture,the purpose is to make its content in order to reach a certain detection,commonly used before the broth with chopped meat medium and trypsin tryptone glucose yeast extract medium(TPGYT),in order to foster identification of Clostridium botulinum base Egg Yolk Agar Base plate and blood culture.The rapid detection method of Clostridium botulinum is 16SrRNA gene matching method,the specific gene RCR amplification detection method.To obtain single colonies of suspected Clostridium botulinum detection by traditional culture,single colonies were obtained after culturing a large number of colonies,to facilitate further rapid detection,so as to confirm whether the suspicious strains of Clostridium botulinum.Through the comparison of 16SrRNA gene,we can get the range of suspicious bacteria,and give the detection direction,which greatly saves the test time.Comparison of the 16SrRNA gene of all the suspected strains showed that not only Clostridium botulinum.Some of the results were compared with Bacillus cereus,Bacillus thuringiensis,Bacillus cereus and Bacillus anthracis,some of which were lactobacillus,some of which were Bacillus licheniformis and Clostridium perfringens.The result of the comparison of 16SrRNA gene is only the name of the strain which is close to that of the suspicious bacteria.According to the range of 16SrRNA gene comparison results,the corresponding RCR method was selected to verify,and a complete set of biochemical tests were carried out if necessary.Finally,the results of the verification and 16SrRNA gene comparison results are basically the same,the test samples were finally detected Clostridium perfringens,Bacillus cereus and lactobacillus.The purpose of this study was to explore the effectiveness of rapid detection methods(PCR,16SrRNA)for Clostridium botulinum and its toxin by a large number of samples.By the final test data,the formula is susceptible to which non Clostridium botulinum belong to other anaerobic bacillus and the pollution,and discuss the security risks of these non Bacillus Clostridium botulinum to eaters.