Research On The Reduction Of U(?) By Two Strains Of Special Bacteria In The Presence Of Photoelectron

Aimed at the study on reduction of U(?)by microorganisms in the presence of photoelectron,S.putrefaciens and R.kocuria bacterias,U(?)was chosen to do the research,in this paper,we studied effect of photoele ctron on the growth and metabolism of S.putrefaciens and R.kocuria,and the U(?)reduction in the single and double chamber,we also analyzed reduction of U(?)by electros mediators of riboflavin and anthraquinone-2-sulfonate in the presence of photoelectron.The results were showed as below.In the range of uv-vis spectrum,the P25 minerals semiconductor electrodes we prepared showed stable and better photoelectric response,with U(?)and H+ decreased,the p H would increased,conductibilit y would decrea sed,and in the U(?)photocatalysis experiment,we fond that,with four hole scavengers added,the U(?)reduction was increased,and formic acid > ethanoic acid > methanol > ethanol,the results indicate that the capacit y of hole scavenger acid low-molecular-weight organics is stronger than alcohol low-molecular-weight organics as high ionizing abilit y of acid low-molecular-weight organics.With 60 mmol/L formic acid added,the 90.26 % U(?)were reduced by photoelectron,and electrochemical impedance spectroscopy(EIS)analysis indicated that P25 mineral semiconductor electrode exhibits higher electron transport resistance abilit y after photocatalytic reaction than before.SEM-EDS results showed that some squares of uranium minerals was showed up after photocatalytic reaction,which adsorbs on the electrode surface and occupied act ive site of TiO2.We also analysed the reduction of U(?)in single and double chamber system,and we found U(?)reduction rate which in single chamber system was higher than which in double chamber system,which indicated that P25 mineral semiconductor electrode also could reduce U(?)in single chamber system.For the study of U(?)reduction by photoelectron in the presence of RF and AQS,we found that electron shuttle first inhibited reduction of U(?),then promoted,but with AQS added the reduction of U(?)was higher than RF,and reduction reaction production of U(?)was same with AQS and RF added,but we found that reduction reaction production with AQS added was easier adsorbed on the electrode,EIS analysis also indicated that electrode exhibits lower electron transport resistance abilit y after photocatalytic reaction than before.In the double chamber system of S.putrefaciens and R.kocuria bacterias grow in the presence of photoelectron,photoelectron could stimulated the grow of S.putrefaciens and R.kocuria bacterias,and R.kocuria bacterias growed better.In the the system of U(?)reduction by photoelectron in the presence of S.putrefaciens and R.kocuria bacterias,the R.kocuria bacterias also growed better,and the S.putrefaciens and R.kocuria could increasd the reduction of U(?),which indicated that two strains of bacterize could increase the reduction of U(?),and R.kocuria showed better.In the double chamber system,While bacterias as the Center,the U(?)reduction reaction production was decreased with distance increased.In the double chamber system of S.putrefaciens and R.kocuria bacterias grow in the presence of photoelectron,the stretching vibration peak and anti vibration peak of Two strains bacterize phosphate diester groups was offset to high wavenumber,the C=O Group stretching vibration absorption of S.putrefaciens porotein amide and symmetric vibration peak of S.putrefaciens polysaccharide molecular methyl was offset to low wavenumber,the same situation was happened to R.kocuria,but the offset of S.putrefaciens was more than R.kocuria,which indicated that the specific hydrogen bonding structure of secondary structural conformation in S.putrefaciens amide I band protein was easier to be destroyed than R.kocuria.