Extraction Of Moringa Polysaccharides By Ultrasonic Combined With Synergistic Enzyme

In order to improve the extraction efficiency of Moringa oleifera polysaccharide,this paper presents a new extraction process:ultrasonic synergistic enzyme.The extraction process,extraction kinetics,separation and purification,physicochemical properties and in vitro antioxidant activity of Moringa oleifera polysaccharide were investigated.Moringa Polysaccharides were extracted by hot water,ultrasonic,and ultrasonic synergistic compound enzyme and the extraction of Moringa powder were studied.The optimum technological parameters were obtained by single factor experiment and orthogonal experiment:the ratio of material to liquid was 1:80,100 ?,and the yield of crude polysaccharide was 7.86%.The yield of crude polysaccharide was 7.86%The optimum extraction conditions were as follows:ultrasonic time 40 min,temperature 50 ?,ratio of solid to liquid 1:100,the yield of crude polysaccharide up to 18.12%.The optimum technological parameters were as follows:ultrasonic frequency 20 kHz,ultrasonic power 180W,ultrasonic temperature 50 ?,ultrasonic extraction 30 min cellulase 1800 U · mg-1,protease 4400 U · mg-1,fruit The extraction temperature was 75 ?,the extraction time was 55 min,the pH was 4.5,and the yield of crude polysaccharide was 33.03%.The optimal technological parameters of ultrasonic and cellulase extraction were as follows:the ratio of material to liquid:1:50,pH 5.17,ultrasonic power 210 W,ultrasonic frequency 20 kHz extraction time 35min,extraction temperature 75 ?,fiber Primer 1800 U · mg-1 was added at the same time,and the extraction rate of polysaccharide was 33.11%.The fibers of the untreated mauve leaf powder were observed by scanning electron microscopy,and the fibers were attached to the fibers.The fiber bundles after pretreatment with ethanol did not change obviously.After hot water reflux,The surface of the fiber bundle was smooth and intact.The fiber bundles appeared on the surface of the cell wall,and the cell wall appeared when the ultrasonic fiber was extracted and extracted by the compound enzyme.On the basis of the optimum process parameters,the kinetic equation,apparent rate constant,apparent diffusion coefficient,apparent rate constant,The extraction rate and the extraction rate of the solution were changed with time under different ultrasonic power,extraction temperature and pH.The empirical formula was proposed to reflect the difference between the apparent diffusion coefficient and the ultrasonic power,temperature and pH during the extraction of ultrasonic synergistic complex Function relations,and the model parameters were verified.The results showed that the extraction rate of superconcentrous compound enzyme was increased by 48.87%compared with that of hot water reflux method at 30 min after 30 min of hot water refluxing and ultrasonic synergistic compound enzyme extraction.The PSMO was obtained by deproteinization,decolorization and dialysis,and the polysaccharides were extracted by DEAE-52 cellulose column to obtain three kinds of polysaccharides,0.05 mol L-1 NaCl of the eluted fraction of PSM1,0.1 mol L-1 NaCl of the eluted fraction of PSM2,0.15 mol·L-1 NaCl of the eluted fraction of PSM3.PSM2 was concentrated under reduced pressure,dialyzed and lyophilized,and then separated by Sephadex G-100 to obtain a elution peak named PSM2S.The results of UV and IR spectroscopy showed that PSM2 was purified by DEAE-32 cellulose column and Sephades G-100 column with high purity and high molecular absorption peak.The antioxidant activity of PSMO,PSM1,PSM2 and PSM3 were studied.The scavenging ability of scavenging polysaccharide to hydroxyl radical,superoxide anion and DPPH was as follows:DPPH free radical and superoxide anion radical:PSMO>PSM2>PSM3>PSM1;hydroxyl radical:PSM0>PSM3>PSM2>PSM1.