Study On Characterization Of Neurospora Crassa Spores And Its Digestion In Vitro

In this paper the research group from screening-Neurospora crassa.as the research object,to detect Neurospora crassa spore and spore spore wall composition and physicochemical properties,response surface method is used to extraction of?-carotene with HPLC-DAD optimized Neurospora crassa spores,change of composition and antioxidant activity in vitro and in the process of storing the Neurospora crassa spores of carotenoids are discussed,and the carotenoid production by fermentation characteristics in vitro digestion after vitro fermentation.The main results are as follows:1,The main component of Neurospora crassa spore and spore wall for water,crude fiber,crude fat,protein and chitin.The spores in water,crude fiber,crude fat,protein and chitin content were 17.18%,29.91%,0.31%,28.32% and 0.66%;the spore wall in water,crude fiber,crude fat,protein and chitin content were 8.74%,31.62%,0.79%,38.38% and 1.19.The main component of hydrolysis of the spore wall polysaccharides of Arabia sugar,Xylose,Mannose,Glucose and Galactose,with reference to domestic research,validation of spore wall polysaccharides mainly divided into Glucan and Glucomannan.Comparison of fatty acids,found in spore in the highest content of unsaturated fatty acids,fatty acids accounted for more than half;saturated fatty acid content of the spore wall in the highest,accounting for more than half of the spores of fatty acids.The physicochemical properties showed Neurospora crassa spores are spherical or ovoid,and the state of aggregation,the diameter values in the 4-20?m and the spores in aqueous solution,the solubility of acetone and ethanol solutions are not ideal.The production of Neurospora crassa carotenoid HPLC analysis found that the content of lycopene and beta carotene respectively19.49?g/g and 155.91?g/g.2,The rough shaped veins of conidia in ?-carotene extraction from optimization,single factor experiments showed that using acetone as extraction solvent,extraction temperature of 50?,extraction time 60 min,ratio of material to liquid 1:100 under the condition of high amount of extract of ?-carotene.The response surface experiment to ensure further the optimum extraction conditions of pigment ?-carotene,?-carotene in acetone as extraction solvent,extraction temperature of 54?,extraction time 59.2min,ratio of material to liquid extraction under the condition of 1:100 is the highest,up to 561.082?g/g.Spores found in experiments,optimization of extraction conditions after high and close to the predicted value,indicating better fitting degree of the model.3,After in vitro digestion,carotenoid release rate for mouth < stomach<small intestine,kind of carotene antioxidant activity for mouth < stomach<small intestine,4? and dark storage conditions most conducive to the protection of Neurospora crassa in carotenoids and carotenoid content of the least loss of antioxidant the decreased activity of at least,dark condition,Neurospora crassa is not conducive to the protection of the carotenoid antioxidant activity,the antioxidant activity decreased most.Neurospora crassa spore wall in vitro digestion process of carotenoids released only 32.58%,so we can protect the good stability of spore in carotenoids in digestive fluid under the action of.4,Neurospora crassa carotenoid production after in vitro digestion of the vitro fermentation,experiment of fermentation in 18 h,with the increase of fermentation time,the gas production increased gradually,each volume fermentation after 18 h were stabilized.In vitro fermentation 24 h,p H values in each experimental group the trend of change is consistent,both decreased first and then increased after 24 h fermentation in each experimental group p H value tended to be about 6.0-7.0,close to the human intestinal p H value.In vitro fermentation 24 h in each experimental group NH3-N content change trend line,were first increased and then decreased and then increased,after 24 h fermentation experiment group NH3-N were compared with the initial fermentation increased.In vitro fermentation 24 h,the content of acetic acid groups were increased first and then decreased slowly,the propionic acid content increased,butyric acid content was first increased and then decreased slowly.After digestion of spore fermentation For inspection found that the intestinal flora,fully digested the spore powder on human intestinal flora disturbance is small diversity,little influence on the stability of human intestinal environment,conducive to the improvement of human intestinal microflora,optimization of each bacterium in intestinal flora proportion,beneficial to human intestinal health.