Synthesis And Properties Of Molecularly Imprinted Polymer Via Visible Light Activated RAFT Polymerization In Aqueous Media At Room Temperature

Recently decades,Despit of the rapid development of imprinted polymers in biological medicine,many challenges have yet to be solved.For example,the complexes of template molecules and functional monomers self-assembled via hydrogen bonding or electrostatic interactions in high-temperature environments may still be affected by the polymerization temperature.The mild environment where most proteins and antibodies in living systems are prepared has inspired fascination among chemists.In addition,the bio-macromolecule is usually synthesized under mild conditions in vivo,such as protein.Therefore,developing a fast,controllable,hydrophilic molecularly imprinted polymer with low carbon and mild conditions will be of great significance in the environment,food and biomedical applications.Without heating during polymerization,visible light activated RAFT polymerization is one of the living/controllable polymerization processes,and it is an efficient and fast low carbon polymerization method.Selecting glucose as template molecules,We are the first attempt to prepare MIPs via visible light activated RAFT precipitation polymerization in aqueous media at room temperature,and the chemical and physical stability of molecularly imprinted electrochemical sensors are investigated.The implement process is as follows:(1)The preparation of MIPs: The prepared conditions of MIPs are optimized with monomer description,such as acrylic acid,acrylamide and BIS acrylamide.While the BIS acrylamide is selected as optimum function monomer for glucose.Subsequently,the optimum ratio design of template,monomer and cross-linker is performed.Comparison with the current response sensitivity of the MIP sensors for[Fe(CN)6]3-/4-oxidation peak by fixing the template,i.e.,glucose,at 0.10 mM,the appropriate ratio is 1:3:9(glucose/DAAM/EGDMA).(2)The synthesis and characterization of MIPs: The proposed Glucose-MIPs using EDMAT as CTA,TPO as photo-initiator,glucose as template,DAAM as double functional monomer,EGDMA as cross-linker,and methanol and deionized water as solvent,is obtained via visible light activated RAFT precipitate polymerization in aqueous media at room temperature.The chemical structure and physical properties of MIPs are characterized by FT-IR spectra,scanning electron microscope(SEM),Dynamic light scattering(DLS)and Brunauer–Emmett–Teller(BET),the results validates this MIPs with micro-sphere morphology,defineddistribution,large surface area,uniform large pore sizes,and high pore volume.(3)Characterization of the MIP sensor: Taking the Glucose as the research object,The performance of molecular imprinting electrochemical sensor is reflected by dynamics adsorption,isothermal adsorption,selectivity adsorption,stability test,and adding sample test in human urine samples.The results indicate that the dynamics process belongs to the pseudo-second model which is chemical adsorption(hydrogen bond),and the adsorption model is attributed to Langmuir which is the single molecular adsorption theory.The MIPs sensor has high selectivity for Glucose and good repeatability.The result of detection of Glucose in human urine samples states clearly that this MIPs sensor can be used to detection of glucose in complicated biological samples such as human urine.In a word,we construct successfully MIPs via visible light activated RAFT precipitation polymerization in aqueous media at room with high selectivity and excellent adsorption kinetics.The experiment results display that it not only achieves the construction of MIPs under the low carbon and mild condition,but also extends the application fields of visible light activated RAFT polymerization at room temperature.