| Based on the non-renewable characteristic of fossil fuels and the strategy of sustainable development, more and more scientists have developed bioenergy to replace fossil fuels, in which hydrogen energy stand out with its high calorific value and by-product only water. Bio-hydrogen technology is a low-cost, low-polluting technology, it has two advantages, the treatment of organic wastewater and clean energy respectively.In this paper, the immobilized technique was applied to biohydrogen technology, the mixed culture of Bacillus cereus A1 and Brevumdimonas naejangsanensis B1, which were selected from the activated sludge, were investigated. Firstly, we chose three natural materials as immobilized carriers, activated carbon 1, activated carbon 2 and corn stalk respectively.The best carrier was corn stalk with the biomass loading of 90.4 mg·g-1 and the yield of hydrogen reached 1.86 mol H2·(mol glucose)-1.In order to improve the hydrogen production efficiency of batch fermentation, the amount of carrier, immobilization mode, and pretreatment of carrier and pH control were optimized. The results showed that 1% untreated corn stalks were used as the best carrier, and the immobilized model was respective immobilization, and the pH maintained at 4.5, in this way, the hydrogen production efficiency was best, the yield of hydrogen production was 1.97 mol H2·(mol glucose)-1.Finally, in order to achieve continuous biohydrogen fermentation, the effects of different HRTs (144 h, 96 h, and 48 h) on semi-continuous fermentation were investigated in this paper. The result showed that when HRT was 48 h, the system could run steadily for more than 20 days and the hydrogen production efficiency was highest. So we selected HRT=48 h for two-stage continuous fermentation, the experimental results showed that the system could run steadily for more than 18 days and the hydrogen production reached 400 mL·d-1, which laid the foundation for the continuous fermentation of mixed strains. |
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