Rare Earth Ions-doped Zinc Based Nanomaterial For Fluorescent Biolabeling

Zinc based nanomaterials are widely used in biodetections and nanotechnology because of their non-toxic and biocompatibility.In this paper,the biofunctional rare earth doped zinc based nanomaterials were synthesized,and the luminescence intensity were significantly enhanced by using the rare earth ion as activator.Then,a series of studies on the luminescence and fluorescent properties of the biolabeling function of rare earth doped zinc based nanomaterials were carried out,The main researches are as follows:?1?Ce³⁺doped nanomaterials based on different matrix zinc were prepared.It is found that ZnS:Ce nanoparticles with cubic ?-ZnS structure have a larger probability of electric dipole transition than ZnO:Ce with hexagonal wurtzite structure.In addition,the ZnS:Ce nanomaterials has a stronger characteristic luminescence of Ce³⁺ ions.?2?Eu³⁺-doped zinc based nanomaterials which have excellent luminescence were prepared by aqueous synthesis methods The red emissions of Eu³⁺ ions was observed under 532 nm excitation.The characteristic emission of Eu³⁺ ions was observed at 579 nm,595 nm,617 nm,650 nm and 714 nm,respectively,and the strongest emission peak at 617 nm is attributed to electric dipole transition arises from the ⁵D₀?⁷F₂.?3?The optimal luminescence of rare earth doped zinc based nanomaterial was used as the probe signal to realize sensitive immunoassay.Human immunoglobulin detected by the photoluminescence spectra of ZnS:Eu as fluorescent probe signal with high specificity and selectivity,and the detection limit is about 10 nM.