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Microbial Degradation Of Organophosphorus Pesticides In Fermented Foods

Posted on:2018-02-06Degree:MasterType:Thesis
Country:ChinaCandidate:Z WangFull Text:PDF
GTID:2321330536457732Subject:Biological engineering
Abstract/Summary:PDF Full Text Request
Organophosphorus pesticides in agricultural production is widely used,it is the result of the phenomenon of pesticide abuse.Pesticide abuse can cause pesticide residues,seriously affecting the safety of food.In this paper,the degradation of several organophosphorus pesticides and their relationship with phosphatase were studied.In addition,the degradation kinetics of organophosphorus pesticides during food fermentation was studied.The content of organophosphorus pesticides in yoghourt of lactic acid bacteria and skimmed yoghourt were determined by liquid-liquid extraction and gas chromatographic analysis in 5 to 20 hours.This test has a pesticide residue level.In addition,the degradation kinetics of lactic acid bacteria was studied by degradation kinetics constants.Secondly,5 to 20 hours after fermentation of yoghurt or lactic acid bacteria,the lactic acid bacteria degradation was further studied by determining the phosphatase activity The effect of pesticide and its relationship with phosphatase;Thirdly,the degradation of organophosphorus pesticides was analyzed by observing the changes of pesticide residues during the fermentation process by liquid-liquid extraction or solvent extraction.In this paper,the related parameters of degradation kinetics of organophosphorus pesticides were studied to analyze the effects of different microorganisms and fermentation processes on food safety.According to this research method,the relevant data are as follows:(1)lactic acid bacteria in skim milk to promote the degradation of organophosphorus pesticides.It can be concluded that the addition of lactic acid bacteria and starter to some extent can effectively improve the degradation rate of organophosphorus pesticides and improve the safety of food.According to the kinetic constants of degradation of organophosphorus pesticides,it was concluded that the promoting effect of starter on degradation was stronger than that of lactic acid bacteria.(2)The ability of lactic acid bacteria to degrade organophosphorus pesticides is related to the content and activity of phosphatase.It was found that the higher the activity of phosphatase was,the stronger the effect of lactic acid bacteria on the degradation of organophosphorus pesticides was.Therefore,lactic acid bacteria and skim yogurt fermentation enzyme phosphatase activity is higher,the degradation of organophosphorus pesticides to promote the more obvious.(3)Microbial fermentation of organophosphorus pesticide degradation has a catalytic role.The addition of fermenting agent increased the degradation kinetics constants of organophosphorus pesticides.Organophosphorus pesticide degradation rate in different foods are different,different types of organophosphorus pesticides,and its stability are not the same.In the process of fermented food production,food microbes can promote the degradation of organophosphorus pesticides,which can effectively avoid the pesticide residues in food and provide the guarantee for food safety.
Keywords/Search Tags:Organophosphorus pesticides, lactic acid bacteria, microbial fermentation, microbial degradation
PDF Full Text Request
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