Biomimetic Synthesis Of Bone-like Apatite On The Surface Of PPBESK

Poly(aryl ether)s have presented outstanding combination properties.They have important polymeric materials universally applied in automobile,petrochemical,aerial industry,electric engineering,nuclear energy,etc.Because of the exceptional mechanical properties,suitable density,radiolucency,and chemical stability,etc,poly(aryl ether)s have more and more used in biomaterials such as trauma,orthopedic,and spinal implants.But the poly(aryl ether)sbiomaterials' high price and poor biocompatibilityresult a bad influence on the development of these materials.In our research group,we develop a series copoly(aryl ether)s containing phthalazinone moieties,which have good solubility and lower price.On the other hand,bone-like apatite is coated on the surface of copoly(arylethersulfoneketone)containing phthalazinone moieties(PPBESK)by using biomimetic synthesis,which will improve the biocompatibility.With spin coating,PPBESK film was coated evenly on the silicon surface.Then polydopamine(PDA)layer was created to induce the formation of bone-like apatite.At the same time,phosphate group was also added on the surface of PDA,aiming at increasing the efficient of mineralization.The thickness,hydrophilicity,surface energy,roughness,chemical component,surface topography,and cytocompatibilitywere all characterized.The thickness of PPBESK film on silicon and PDA layerwas 53 nm and 33 nm respectively.Because of PDA,the surface of material became much rougher.At the same time,contact angle of PPBESK/PDA coatingwas 47.7°,compared with 88.3° before modification.Despite the reducing the thickness of PDA and roughness,phosphate group increased the efficiency of mineralization.And the layer of apatite was around 10?m.Meanwhile,cytotoxicity,cell adhesive ability,cell apotosis,alkaline phosphatase(ALP)activity assayand real-time PCR analysis by the cell of MC3T3-E1 Subclone 14 demonstrated that samples modified by bone-like apatite exhibited good cytocompatibility and osteogenesis differentiation ability.On the basis of above studies,injection molding PPBESK's mechanical properties was detected,reaching the level of biopoly(aryletherketone).Then bone-like apatite was introduced on the surface of PPBESKthough PDA.Hydrophilicity,roughness,chemical component,surface topography,and cytocompatibilitywere all investigated.The result showed that: PDA layer can be stable on the surface of the PPBESK.Contact angle of PPBESK/PDA coating was 29.3°,compared with 87.7° before modification.And the surface of material became much rougher.The hydrophilicityand cytocompatibility of PPBESK coated with bone-like apatite wereall improved greatly.