Study On The Fabrication Of Zn-doped Micro/Nano-textured Surface And Its Biocompatibility

Although the early study focused on fabricating the well-ordered mesoporous TiO₂ micro/nano textured coating in nitrate electrolyte under high current anodization,which is more beneficial to promote osteointegration.However,the active elements cannot been loaded into the coating during the process of high current anodization,leading to a lower bioactivity of the coating.Based on this,we choose zinc nitrate as the main ingredient of the electrolyte,through high current anodization,the micro/nano textured TiO₂ composite coating can be prepared on the titanium-base material surface,and further,by adjusting the pH of the electrolyte,the zinc elements can be doped into the surface of the coating.Furthermore,the osteoblast is used as in vitro model to evaluate the effect of modified surface on the adhesion,proliferation and cell functions of cultured osteoblasts.?1?A special layer composed of mesoporous is fabricated on the Ti surface by the high current anodizing method.The mesoporous arrays present a honeycomb-like structure.Compared to the nanotubes,the mechanical properties of the oxide layer is significantly improved.?2?It is proved that the NO3-plays significant mediation role in the formation of TiO₂ mesoporous arrays structure during the process of anodizing..?3?With the increasing of current density,the surface roughness and the size of mesoporous are significantly increased.It is proved that the well-organized mesoporous arrays can be fabricated when the voltage exceeds 30 V.?4?The doping of Zn is significantly influenced by the pH value of the electrolyte.The Zn is detected as the form of ZnO within the formed oxide layer.?5?The released Zn ion can promote cell spreading,cell proliferation,and the ALP activity of cells.It is proved that the released amount of Zn exceeding 10.5 ?g/cm2*day may lead to cytotoxicity.