Study On The Application Of Metal-Organic Frameworks And Its Derivative Materials As Active Peroxidase Mimetic

Enzymes participate in many important life activities in the organism.Natural enzymes have the advantages of mild reaction conditions,high catalytic efficiency,good specificity and good selectivity.They are widely used in many fields such as biology,medicine,chemical industry and so on.However,the natural enzyme has some limitations,such as easy to inactivate,volatile,poor stability and so on.And natural enzyme has a high cost on preparation,purification and storage.Therefore,it is necessary to produce a well-behaved mimetic enzyme.Glucose is a common metabolic intermediate and major energy source in living cells and biological systems.And glucose can provide basic information on many diseases such as hypoglycemia and diabetes.In recent years,the peroxide mimetic enzyme in the form of nanomaterials has received considerable attention in the detection of glucose and hydrogen peroxide applications.So it is important to design a mimetic enzyme with high catalytic activity,good selectivity,low cost and good stability.Metal organic frameworks?MOFs?has a high surface area,porous,rich and open metal active sites,but little research has been done with MOFs as a mimetic enzyme.Based on this,this paper carries out research work on the development and research of new MOFs and its derivatives as a peroxide mimetic enzyme,the construction of nanomaterials to enhance the catalytic activity of peroxide mimetic enzymes and the application of peroxide mimetic enzymes in the study of chemiluminescence analysis.The paper is divided into four chapters:Chapter 1: It is literature Review.Mainly in recent years on the MOFs and other nano-materials as a peroxide research enzyme research progress.Chapter 2: In this chapter,the water stability MOFs Fe-MIL-88NH₂ with rich open iron metal sites was synthesized by microwave method to detect H₂O₂ and glucose as high efficiency mimetic enzymes.The results of chemical kinetics and chemiluminescence spectra show that MOFsFe-MIL-88NH₂ can effectively enhance the luminescence of the luminol-H₂O₂ weakly chemiluminescent system.We conclude that the enhancement of chemiluminescence signals may depend on the electron transfer process in chemiluminescence systems and free radicals produced by the decomposition of hydrogen peroxide catalyzed by MOFs Fe-MIL-88NH₂ nanomaterials.Based on hydrogen peroxide concentration and material MOFs Fe-MIL-88NH₂ nanometer catalytic activity,to establish a simple,sensitive and relatively good selective chemiluminescence analysis determination of hydrogen peroxide in environmental measuring water samples.If the glucose oxidase catalytic reaction is disturbed,a chemiluminescence assay for the indirect determination of glucose can be established.Under the optimum experimental conditions,the linear range of H₂O₂ is1×10-7 mol / L to 2×10-5 mol / L,and the corresponding detection limit is 1.3×10-8 mol / L.Under the optimum conditions,the glucose concentration in the range of 1×0-7 1×10-5 mol / L showed a good linear relationship with the corresponding chemiluminescence intensity.The results show that the method can be successfully applied to the determination hydrogen peroxide in the lake and Glucose in serum samples.Chapter 3: The PVP-modified MOFs Fe-MIL-88NH₂ was synthesized by microwave as a highly effective mimetic enzyme to detect H₂O₂ and glucose.The results ofchemical kinetics and chemiluminescence spectra show thatMOFs Fe-MIL-88NH₂ can greatly enhance the luminescence of the luminol-H₂02 weakly chemiluminescent system.We conclude that the enhancement of chemiluminescence signals may depend on the electron transfer process in chemiluminescence systems and free radicals produced by the decomposition of hydrogen peroxide catalyzed by MOFs Fe-MIL-88NH₂ nanomaterials.The material catalyzes the decomposition of hydrogen peroxide to produce hydroxyl radicals and superoxide radicals.The resulting reactive oxygen free radicals induce the luminescence of the excited state of 3-amino-phthalic acid anion free radicals,and when returned to the ground state,produce luminescence.Due to the correlation between the concentration of hydrogen peroxide and the catalytic activity of the material,it is possible to establish a simple,sensitive and relatively selective selective chemiluminescence assay for the determination of hydrogen peroxide in environmental water samples.If the glucose oxidase catalytic reaction is disturbed,a chemiluminescence assay for the indirect determination of glucose can be established.Under the optimum experimental conditions,the linear range of H₂O₂ was 5 × 10-8 mol / L to 1 × 10-4mol / L.Under the optimum conditions,the glucose concentration in the range of 1 × 10-8 1 × 10-5 mol / L showed a good linear relationship with the corresponding chemiluminescence intensity.The results show that the method can be successfully applied to the determination hydrogen peroxide in the lake and Glucose in serum samples.Chapter 4: The composite materials of ZIF-67 MOFs modified with carbonized PVP and PVP-modified MOFs Fe-MIL-88NH₂ were used as high performance mimetic enzymes to detect H₂O₂ and glucose.The results of chemical kinetics and chemiluminescence spectra show that the carbonized composite can greatly enhance the luminescence of the luminol-H₂O₂ weakly chemiluminescent system.We conclude that the enhancement of chemiluminescence signals may depend on the electron transfer process in chemiluminescence systems and free radicals produced by the decomposition of hydrogen peroxide catalyzed by MOFs Fe-MIL-88NH₂ nanomaterials.The material catalyzes the decomposition of hydrogen peroxide to produce hydroxyl radicals and superoxide radicals.The resulting reactive oxygen free radicals induce the luminescence of the excited state of 3-amino-phthalic acid anion free radicals,and when returned to the ground state,produce luminescence.Due to the correlation between the concentration of hydrogen peroxide and the catalytic activity of the material,it is possible to establish a simple,sensitive and relatively selective selective chemiluminescence assay for the determination of hydrogen peroxide in environmental water samples.If the glucose oxidase catalytic reaction is disturbed,a chemiluminescence assay for the indirect determination of glucose can be established.Under the optimum experimental conditions,the linear range of H₂O₂ was 1×10-8 mol / L to 6×10-5 mol / L.under the optimum conditions,the glucose concentration in the range of 5×10-8 3×10-5 mol / L showed a good linear relationship with the corresponding chemiluminescence intensity.The results show that the method can be successfully applied to the determination hydrogen peroxide in the lake and Glucose in serum samples.