Fabrication Of Antioxidant Pickering High Internal Emulsions And Porous Materials By Gliadin Colloid Particles

Gliadin is the main storage protein of wheat endosperm,gliadin is a kind of amphipathic and unique self-assembly characteristics,hydrophobicity and good biocompatibility.The self assembled gliadin colloid particles were used as emulsifiers,as to stable food grade Pickering HIPEs,and by manipulating the interface layer structure of antioxidant Pickering HIPEs.Used the Pickering with high internal phase emulsions as templates to prepare a new protein based porous material,this material has a good prospect in porous catalysis and adsorption,the main results are as follows:1.The composite colloidal particles(GCHP)of Gliadin and Chitosan were prepared by anti-solvent method,and their related changes were investigated by adjusting the pH value of GCHP.The interfacial adsorption and rheological properties of the composite colloidal particles were studied by interfacial adsorption kinetics and interfacial rheology then analyzed the adsorption characteristics of the composite colloidal particles.Gliadin has high load and encapsulation ability for biologically active substances,and the interaction between gliadin and Curcumin is studied by fluorescence quenching.Research shows,changes in pH have an effect on GCHP surface wettability,GCHP adsorption in the oil-water interface is formed by the elasticity of the viscoelastic interface,the interaction between gliadin and curcumin is mainly through hydrogen bonding.2.Fabrication of composite colloidal particles loading Curcumin(GCHP-Cur)by Anti-solvent Technology.Explore the effective formation pathways of composite colloidal particles stable antioxidant Pickering high internal phase emulsions,research and reveal its formation mechanism,and by manipulating the interfacial layer structure fabrication antioxidant Pickering High Internal Emulsion,then established the relationship between the oxidation stability of the emulsion and the interface layer structure and the micro-distribution of the antioxidant.Research shows,loaded the curcumin did not change the ability of colloidal particles to stabilize the emulsion,the prepared Pickering High Internal Emulsion has excellent stability and emulsion is a gel-like emulsion with elasticity.Through the CLSM study found that curcumin was successfully brought to the emulsion oil-water interface,at the same time,curcumin is located in the oil-water interface with better antioxidant properties were found.This provides a new way to solve the problem of unsaturated grease oxidation by manipulating the emulsion interface structure.3.GCHP as the water phase and n-hexane as the oil phase,combined with freeze-drying technology to prepare a new type of porous materials.The effects of different GCHP concentrations and different oil phase ratio on the porous materials were systematically studied.The internal structure morphology of the porous materials was studied by scanning electron microscopy(SEM).Research shows,low GCHP concentration(0.5%)and high oil phase ratio(90%)still can fabrication a stable emulsion.It is found that the emulsion is a viscoelastic emulsion with elasticity.Genipin,a biological cross-linking agent,has no effect on the formation and microstructure of Pickering HIPEs,but the Genipin acts as a crosslinking agent for the porous material,and its structure is strengthened.