| In this paper,the essential oil was extracted from rosemary,and carnosic acid was extracted from the deoiling rosemary by alcohol reflux extraction.The purification of carnosic acid was studied by pH controlled precipitation and silica gel column chromatography,exploring a technology route for industrialized production,and rosmarinic acid was enriched in the waste liquid carnosic acid was decolorizated by activated carbon to purify and the antioxidant effect of carnosic acid paste in cosmetics was studied.The results were as follows:1.Determination of components with strong antioxidant acivity in rosemary was confirmed: The conditions of CA: the analysis was carried out on Pheromenex Ecosil C18column(200 mm ×4.6 mm,5 μm),the mobile phase was composed of actonitrile and 0.1%phosphoric acid aqucous(60:40,V/V).The detection wavelength was 230 nm,with flow rate1.0 mL/min at column temperature of 30 ℃,the HPLC detection method shows pefect repeatability and stability between the concentration of CA and peak area with R2 of the standard curves were 0.9992.2.The extraction process for CA from rosemary was established: 80% ethanol extracting with material and solvent ratio of 1:14,extraction temperature in 80℃,extraction for 2 times,70 min each time.Under this conditions,the average extraction rate of CA was up to 91.31%,and the purity of carnosic acid was 21.24%.3.The purification of CA was studied: 20% carnosic acid,ethyl acetate extracting with material and solvent ratio of 1:10,filtration,filtrate was extracting for 2 times with4%NaHCO3(1:1),merge the filtrate,adjust pH decline to 2 with 1% HCl,precipitaton after stewing 24 hours,filtration,residue were dryed by vacuum drying,and the purity of carnosic acid was 62.13%,and the recovery was 81.26%.The purification of CA enriched by precipitation was further purified by silica gel column chromatography.The optimization conditions of silica gel column chromatography were particle sizes of silica 200-300 mesh with 40 g,the volume ratio of eluent ethyl acetate and petroleum ether 4:2,the flow rate 6mL/min and 0.8g solid loaded.The result showed that the purity of CA was 95.69%,and the recovery of CA was 72.85%.4.The decolorization of CA was researched: The optimization of conditions ofdecolorization were ratio of activited-carbon 2%,temperature in 70℃,extraction time for 50 min,extraction pH 5.The result indicated that “△E*ab” was 5.06 and the effect of decolorization was significant under this condition,and recovery is high for 91.50%,which solved the problem that the interference of colour of CA was applicated in the terminal products.5.The antioxidant capacity of CA applicated in cream cosmetics was studied: CA with different concentration was added into cream cosmetic dissolving by EtOH,and then measured the antioxidant capacity of solution of cream cosmetic.The ability to scavenge ABTS radicals was converted to Trolox concentration at the same concentration,the ability of CA(0.0183mg/mL)was greater than TBHQ(0.0161mg/mL),BHT(0.0112mg/mL),BHA(0.0130mg/mL).In FRAP test,the FRAP value was used to characterize the strength of iron reduction,the FRAP of CA was 0.0073mg/mL,which was lower than TBHQ(0.0083mg/mL),but higher than BHA(0.0039mg/mL)and BHT(0.0055mg/m L);In the reducing DPPH radical ability tests,the ability to reducing DPPH radical ability was expressed as SC50,the capacity of different antioxidants sequence: TBHQ>CA>BHA>BHT. |
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