Preparation And Property Research Of Polyphenols Inverse Microemulsion Using Edible Oil As Continuous Phase

Water-solubility characteristic of polyphenols has restricted their application as natural antioxidant in oil industry.In order to reduce the use of synthetic antioxidants and increase the solubility of polyphenols in edible oil,water in oil(W/O)microemulsion(ME)using edible oils as continuous phase to envelop polyphenols was prepared.The phase behavior,electrical conductivity,particle size and distribution,oxidation resistance ability of the ME was investigated by pseudo-phase diagram method,conductivity method,dynamic light scattering(DLS)and differential scanning calorimetry(DSC).The concrete research contents and results are as follows:(1)W/O ME of Polyoxyethylene(10)oleyl ether(Brij97)/ Glyceryl Monooleate(GMO)/ethanol/ soybean oil /water was prepared successfully.Determine the formula of the ME was that the oil phase was 5 kinds of edible oil,the complex surfactant was Brij97 and GMO,the aqueous phase was water and ethanol.The best mass ratio of Brij97 to GMO was 2:8,the best mass ratio of water to ethanol was 4:1.The appearance of W/O ME was a transparent and stable.(2)W/O ME of Brij97/GMO/ethanol/edible oil /water was prepared successfully.The effects of oil phase on the W/O ME region were investigated by pseudo-phase diagram method,the results showed that the different kinds of oil phase had no effect on ME region.Temperature had large effect on the region of ME by the pseudo-ternary phase diagrams,gradually with the temperature increased,system solubilization ability enhanced,and ME region also increased accordingly;salinity had little effect on the region of ME,showing good salt tolerance of this ME.Moreover,conductivity distinguished the phase transition with the addition of water.Last,DLS data indicated that the types of edible oil in the oil phase had no relationship with the mean particle size of ME,and the mean particle size of 5 kinds of ME systems was stable and basically remained stable for 30 days.Salinity had effect on the mean particle size of ME,with the salt concentration in the water increased,the mean particle size of the ME decreased.(3)Brij97/ GMO/ethanol/ edible oil /water ME to envelop tea polyphenols(TPs)was prepared.in this paper,the results showed that the stability of ME was good,and the phenomenon of oil-water separation was not appeared;DLS data indicated that the higher the concentration of TPs,the lower the mean particle size of ME system.In addition,the mean particle size of 5 kinds of ME systems with adding 0.10 g/m L TPs were between 8.00-12.00 nm and basically remained stable for 30 days.The results of DSC showed that the incipient oxidation temperature of blank soybean oil ME was 163.40 ?,and the temperature of ME with adding 0.01 and 0.10 g/mL TPs were 175.77 ? and 210.88 ?.(4)Based on the content of the former three chapters,Brij97/ GMO/ethanol/ edible oil /water ME to envelop Canna edulis Ker polyphenols was prepared successfully.The results showed that the stability of ME was good,and the phenomenon of oil-water separation was not appeared.Moreover,the stability of particle size of the ME systems with adding 0.01 g/m L Canna edulis Ker polyphenols was stable and basically remained stable for 30 days;When the polyphenols concentration was adding to 0.10 g/mL,the demulsification of canola oil and sunflower oil ME were appeared after 20 days.The results of DSC showed that the incipient oxidation temperature of ME with adding 0.10 g/m L Canna edulis Ker polyphenols were 179.63 ?,which was increased 34.90 ? than blank ME.