Preparation Of Wheat Gliadin Nanofibers By Electrospinning And Characteristic

The technology of Electrospinning could be used to prepare nanometer load systems for various bioactive components.The process of preparation was simple,effective,mild and with no need of surface-active agents.During this process,the amount of solvents used was a little,and the natural food-borne high-molecular polymers(such as protein,polysaccharide)could be used as the carriers,expecting to be largely used in the food industry.Gliadin,which could be got easily and cheaply,possesses a high safety and a good degradability.It could be attached to the gastrointestinal mucosal by the mutual function of the hydrogen bond,VDW and hydropHobic bond with the digestive tract mucoproteins.Gliadin could help to increase both the absorptivity and the bioavailability of the active components loaded,tending to be the better substitute for the polymers compounded.In this paper,wheat gliadin nanofibers were prepared by electrospinning,and the process parameters were optimized.DMY-Gliadin nanofibers were prepared by using DMY as a kind of flavonoid compound with multiple physiological activities.And its characteristics were analyzed in order to provide reference for the application of wheat gliadin nano-load system.Wheat gliadin was dissolved in glacial acetic acid and prepared by electrospinning to obtain wheat gliadin nanofibers.The morpHology of the Gliadin nanometers was observed by use of the SEM.With the index of fiber diameter,the effects of Electrosponning speed,Electrosponning voltage,Electrosponning concentration on Gliadin nanometer fiber diameter.By further implementing the response surface design,the optimal technological parameters were obtained as follows: Electrosponning concentration 24%,Electrosponning voltage 10 KV,Electrosponning speed 0.25 mL/h.The morpHology of nanometer fiber prepared under these optimal parameters was in good condition,without obvious beads nor nodule.Also the diameter of nanometer fiber was 131 nm,distributing homogeneously.Moreover,the Electrosponning speed was the most obvious factor effecting the fiber diameter.The DMY ethanol solution was thoroughly mixed with the wheat gliadin acetic acid solution,8% and 12% DMY-Gliadin nanometer fiber were prepared by electrospinning,with the diameters of 596 nm and 199 nm respectively.Their structures were further detected by FT-IR spectrum and Raman spectrum.The results showed that there was no obvious chemical reaction between DMY and wheat gliadin.TG analysis showed that the thermogravimetric trend of DMY-Gliadin nanofibers was similar to that of wheat gliadin at 250 ℃.When the temperature continued to increase with the degradation of wheat gliadin,DMY began to melt and degrade,indicating that the DMY-Gliadin nanometer system The gliadin of wheat has a certain protective effect on DMY.DSC analysis showed that DMY-Gliadin nanofibers disappeared in the DMY endothermic peak,mainly showing the characteristics of Gliadin nanofibers,indicating that DMY has been completely dispersed in DMY-Gliadin nanofibers.The activity and safety of DMY-Gliadin were evaluated.The antioxidant activity was studied by DPPH radical scavenging experiment,hydroxyl radical scavenging experiment and ORAC experiment.The release of deionized water and simulated gastric juice was studied.The results showed that DMY-Gliadin nanofibers had a sustained-release effect on DMY,and its antioxidant effect was gradually reflected with the slow release of DMY.MTT test showed that DMY-Gliadin had no obvious toxicity to LO2 cells.