Fabrication Of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Porous Membranes And Biological Function

Petroleum-based polymer materials are widely used in various fields and play an important role in human life and national economy.However,with the deepening of the energy material crisis and the intensification of white pollution,the sustainable development of bio-based macromolecule materials Favored.Polyhydroxyalkanoats?PHA?is the only polymer material that is completely biodegradable from microbes.At the same time PHA relative to other biological materials?polylactic acid PLA,butylene succinate copolymer PBS,polyglycolic acid PGA,etc.?diversified structure,a wide range of physical and chemical properties are not the same,can be applied to different conditions of application requirements,PHA has become one of the most active research hotspots in the field of biomaterials.The polymer membrane has been widely used in many fields such as particle removal,protein separation and purification,water purification,sewage treatment,hemodialysis and cell porous scaffold.Most of the polymers that can prepare the ultrafiltration membrane are petroleum-based polymer materials,membrane materials dependent on oil energy,and do not have biodegradability after disposal,and are prone to new pollution to the environment,so the development comes from renewable resources and Biodegradable new polymer porous membrane materials are increasingly concerned about,in addition to biocompatible porous membrane in the field of biomedical applications increasingly receive attention.In this paper,Dimethylacetamide?DMAc?was used as the solvent and porous poly?3-hydroxybutyrate-co-3-hydroxyvalerate??PHBV?porous membranes were prepared by nonsolvent induced phase separation.The effects of nonsolvent,polymer concentration,temperature,coagulation bath compositionon and additives the morphologies and performances of PHBV membranes were the investigated.And PHBV membranes with different structures were obtained by controlling the phase behavior.The biocompatibility of poly?3-hydroxybutyrate-co-3-hydroxyvalerate??PHBV?porous membranes was studied by culturing Hela cells with cell membrane culture medium.The studies showed liquid-liquid demixing took place faster in PHBV / DMAc / H₂O than PHBV / DMAc / ethanol at 45?.PHBV membranes prepared in water bath exhibited sponge-like structure and finger-like structure as solvent and nonsolvent exchanged quickly and liquid-liquid demixing happed immediately.While DMAc was introduced into water bath,the exchange rate of solvent and nonsolvent became slower and membranes with uniform sponge-like structure were obtained.The effect of polyvinylpyrrolidone PVP on the pore formation of the porous membrane is not only beneficial to the formation of the porous membrane,but also the honeycomb pores in the honeycomb pores are formed on the lower surface.The pore size distribution is uniform.The addition of the hydrophilic substance PVP is also the increase in the water flux of the porous membrane.At the infiltration temperature of 80 ?,the solidification bath is pure water,the phase separation process,the thermodynamic and kinetic interaction,the PHBV/PVP porous membrane pore formation effect,porosity and water flux is relatively high.The Hela cells were cultured in a Hela cell with a PHBV porous membrane as a cell culture medium,and the V Service value of Hela cells was above 90%.It was proved that PHBV porous membrane was non-toxic and had no effect on cell output.The cells were stained with DAPI dye,and the cell growth and reproduction were observed when the honeycomb PHBV porous membrane was used as the cell culture medium under fluorescence microscope.Therefore PHBV porous membrane for biocompatibility,and can be applied in the field of biomedical.