Ultrasensitive Detection Of Organic Dye In Aquatic Products Using The Coupling Technique Of Dummy Molecular Imprinting And Surface-enhanced Raman Scattering

Pollutants in aquatic products is threatening the healthy of human beings.High sensitive detection of pollutants from water has become a crucial problem in our society.Surface-enhanced Raman scattering(SERS)is an nondestructive and ultra-sensitive detection technology.Recently,SERS is widely used on biomedical detection,single molecule detection,chemical/biological sensor,etc.But lack of pertinence and the SERS signal of the analyte will interfere with other impurities,which limited the development of SERS technology.MIT is a kind of separation technology,which can high recognit template molecule.The coupling technique of molecular imprinting with surface enhanced Raman scattering is regarded as a promising analysis method due to its properties of high sensitivity and selectivity.However,the traditional molecular imprinting technique is very difficult to thoroughly remove template molecules.So,the technique will result in strong background interference impacting the test results when it is combined with the surface-enhanced Raman scattering spectroscopy.In this study,the Ag particles were first synthesized using the method of ascorbic acid reduction.Then,the as-prepared Ag particles were silanized using 3-methacryloxypropyltrimethoxysilane.The dummy molecularly imprinted polymers were synthesized by using abietic acid replacing malachite green as the template molecule to react with methacrylamide functional monomer.The results show that The SERS test proved the limit of detection by Ag particles for malachite green is 10-7 mol/ L.And the resulting ?core-shell? composite substrate has a more remarkable SERS enhancement than Ag substrate.And its detection limit for malachite green can down to 10-11 mol/L.And The dummy molecularly imprinted polymers were synthesized by using abietic acid replacing methylene blue as the template molecule.High sensitive detection of methylene blue The results show that The SERS test proved the limit of detection by Ag@DMIPs particles for methylene blue can down to 10-10 mol/ L.This method can eliminate the background interference and improve the accuracy of the analysis results,which provide a new method for the ultrasensitive detection of organic dyes.