Design And Synthesis Of Novel Small Molecule Fluorescent Probes

With the development of biomedical technology and the urgent need of modern medicine,fluorescence detection technology is widely used because of its low cost,real-time detection,simple operation and so on.At the same time,the response type fluorescent probe is paid more and more attention by the scientific research worker because of its specificity and high sensitivity to the detection substrate.Hydrazine as an important chemical raw materials,in the anti-corrosion,textile dyes,pharmaceuticals,photographic chemicals,emulsifiers and other chemical industry has been widely used.But it is a very toxic substance,not only the environment has a great risk of pollution,the human body also has a strong toxic effect.Therefore,this paper synthesizes Naphpsulf-O,a fluorescent probe,which specifically detects hydrazine with naphthalene as fluorophore.The probe molecule itself has no fluorescence,and it can be used to detect the hydrazine.The sensitivity of the probe Naphpsulf-O to the detection of hydrazine was high,and the detection was up to 22 nmol/L.In addition,the probe on the corpse of the fast response,is conducive to real-time monitoring of hydrazine.More importantly,the probe can achieve quantitative fluorescence imaging of hydrazine in viable cells,which will provide a powerful tool for studying the toxic effects of hydrazine on cells.The probe has a good application in chemical and biological.Therefore,it is possible to detect compound hydrazine in a simple,accurate and in situ manner,which is of great potential value for life sciences,disease diagnosis and environmental science.Reactive sulfur species(RSS)is a common term of sulfur-containing biological molecules,is an important component of the body,in the life of the process has a special physiological function,but also an important material to study the pathological process.Intracellular reactive sulfur species(RSS)such as cysteine(Cys),homocysteine(Hcy),glutathione(GSH)play a huge role in human physiological processes.Therefore,the research and development of highly efficient and sensitive active sulfur species probe is very important to study cell physiology and pathology.In this thesis,based on the mechanism of electron transfer in the excited state molecule,a novel cysteine(CHCQ)was selected as the fluorophore and acrylic acid as the recognition site.A highly selective new Cys fluorescent probes.The response time of Cys,the pH stability of the probe and the specificity of Cys were measured under in vitro physiological conditions.And to investigate the ability of the probe to detect Cys in living cells.In addition,CHCQ-Ac probe has good solubility,low cytotoxicity,and good cell membrane permeability.The CHCQ fluorophore released by the CHCQ-Ac probe and cysteine is insoluble in water and is capable of producing insoluble fluoropolymers that provide intense fluorescence.Thus,the CHCQ-Ac probe can provide a novel,fast-responsive,highly sensitive cysteine assay.