| This paper is divided into two parts.Since their discovery,antibiotics has contributed greatly to human health and social development.With the increasing use of antibiotics,however,its abuse in clinical treatment is also growing.The abuse of antibiotics could not only cause great harm to patients,but also cause potential hazards to the healthy.Therefore,the norms and purification of antibiotic has aroused widespread concern at home and abroad.Up to now,the types of antibiotics has reached thousands of species,commonly used in clinical hundreds of species,which are classified as follows:nitroimidazole,?-lactam,aminoglycosides and so on thirteen species.Among them,nitroimidazoles include ornidazole,tinidazole and so on.First part:the preparation of the conducting polymer-silicone polycondensate duplex molecularly imprinted membrane-based sensor and its application in recogniting S-ornidazole?S-ONZ?.Firstly,3-?phenylamino?trimethoxysilane and?2-Aminophenyl?boronic acid electric benzene monomer,is deposited on the surface of carbon paste electrodes,followed by the electropolymerzation of Si?OCH3?3 and B???grouppolyanilineconductivefilm,andthenaminopropyl trimethoxysilane?as a functional monomer?and tetraethoxysilane?as a crosslinking agent?in the presence of a template molecule S–ONZ were condensed and form a siloxane polycondensate molecularly imprinted membrane vis a sol-gel process over the conductive film.The introduction of B???embedded in introduced Lewis acid into the imprinted site,and the resulting coordination interaction makes the imprinting site with stronger recombination ability for the template molecule and higher enantioselectivity selectivity.In optimal conditions,the sensor S–ONZ had a linear detection range of 6.0×10-8 to 3.5×10-4mol L-1,the detection limit is 1.1×10-8 mol L-1.For detecting S-ONZ in ornidazole tablets human serum and urine content recovery was 95.05-105.95%,RSD of 2.25-4.11%.Second Part:Electrochemical sensor for the determination of tinidazole,This sensor was based on boron embedded molecular imprinting composite membrane?B-MICM?modified multi-walled carbon nanotube?MWCNTs?carbon paste electrode?CPE?.Firstly,the boron-embedded double molecular imprinting membrane?B-MICM?is electrically polymerized onto the surface of a carbon paste electrode?CPE?covered with multi-walled carbon nanotubes?MWCNT s?,and then the molecularly imprinted polysiloxane film?MIP?is hydrolyzed and formed molecularly imprinted layer on the surface of the conductive copolymer layer.Compared with the bare electrode?CPE?,the reduction peak potential of tinidazole on?B-MICM/MWCNT s/CPE?was about300m V and the peak current was increased by 13 times,indicating that significant synergistic effect between B-MICM and MWCNT s.More importantly,the introduction of a coordination interaction between the trivalent boron atoms embedded in the B-MICM matrix and the nitrogen atoms in the template molecule tinidazole endows the sensor affinity and directionality.Then,the electrochemical reduction mechanism of tinidazole was studied by different voltammetry method.Finally,a highly sensitive electrochemical analysis method was developed and used to detect tinidazole in tablets and biological samples.This method has good precision?RSD,1.36%-3.85%?and acceptable recovery?82.40%-104.0%?. |
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