Synthesis And Biological Applications Of Fluorescent Nobel Metal Nanoclusters

Nowadays we all know that cancer(malignant tumor)is one of the major diseases seriously harm to human health.According to the statistics,there are 14.1 million new cases of cancer around world in 2015,and 8.2 million patients were dead from it.Clinical data show that early diagnosis and treatment of tumors can greatly improve the therapy efficiency of cancer.So it is urgent to determine the early stage of tumor to reduce pain and costs of patients,and increase survival rate of patients.But the early symptom of cancer is not obvious,and the routine pathological diagnosis now costs a lot of money and time,accomapnying with low accuracy.So it is of great significance to find a convenient,quick and accurate method of cancer early diagnosis.Because of its special properties,nanomaterial attracts much attention in cancer detection.Especially the noble metals such as gold and silver nanomaterial,which have biocompatibility,stable fluorescence features,has found a broad application in biomedical areas.For example,in the early diagnosis of cancer,nanomaterial can be applied in analysis and molecular imaging of tumor.We know that early diagnosis is important to cancer treatment,so it has a great value to use these metal nanoparticles to realize the valid inspection diagnosis of cancer,and this will eventually make the potential social and economic benefits.In this thesis,a simple and rapid synthesis method of AuAgNCs with fluorescent properties was successfully prepared,and the interaction between AuAgNCs and cancer cells or normal cells was further explored.Meanwhile,the as-prepared AuAgNCs were also applied to the in vivo tumor imaging.The relevant research study is illustrated as follows:1)Fluorescent AuAgNCs nanoclusters were synthesized,which exhibit good fluorescence properties and high stability.The as-formed AuAgNCs nanoclusters were further applied for high sensitive heavy metal ion detection.2)Furthermore,we applied AuAgNCs to cells and in vivo fluorescent imaging,because of its excellent biocompatibility and fluorescent stability.We mainly used HeLa,HepG2,U87 and L02 cells to realize the cell recognition with low toxicity.3)According to the biosynthesis condition of AuAgNCs in situ,we built the model in embryos and juvenile of zebrafish,and explored the in vivo fluorescent imaging efficiency.In this study,we have relized a simple,inexpensive and rapid preparation of AuAgNCs,and applied this good biocompatibility,fluorescent high stability AuAgNCs as fluorescent probes in cells and in vivo imaging.The AuAgNCs have small size,good water solubility,which can readily pass through the tumor angiogenesis with EPR effect.And AuAgNCs has provided a new way of tumor early optical diagnosis because of its good biocompatibility and fluorescent characteristics,and showed potential biomedical applications.