Study On Metabolic Pathways Of Polyphosphate Accumulating Organisms And Glycogen Accumulation Organisms And Its Subgroup

Currently there are many areas of China water environment quality is poor,environmental pollution hazards,water ecological phenomenon such as severely damaged,among them,the growing development of eutrophication of water body is in urgent need of attention and solve the problem of environmental pollution.Biological phosphorus removal technology compared to the chemical phosphorus removal technology avoids the reagent cost of chemical phosphorus removal technology,sediment problem such as environmental pollution,removing effect is better,to improve the utilization of excess sludge can be repeated.Enhanced biological phosphorus removal process,therefore,more and more get the favour of people,as well as the current sewage pollution control and prevention research difficulties and hot spots.This topic proposed in view of the enhanced biological phosphorus removal system and phosphorus accumulating gaos two key flora,the competition between the using SBR technology research,its metabolic pathways by adjusting the pH,temperature,DO,phosphorus,nitrogen,carbon source factors six ecological factor batch experiment was carried out,in order to optimize and control various enhanced biological phosphorus removal system of dephosphorization capability and decontamination performance.In addition,for each phase of the activated sludge of metagenomic high-throughput sequencing,build and phosphorus accumulating gaos gene library and its subgroup,examine microbial community characteristics of SBR system,the relative abundance of species and 16 s rDNA evolved.In the end,provide a reference for optimization of EBPR process operating conditions,to reduce excess sludge production,actual phosphorus removal engineering design put forward reasonable guidance and technical support.In anaerobic-aerobic mode SBR reactor and activated sludge of municipal sewage factory as a kind of mud,after laboratory for 20 d of sludge domestication and cultivation,successful rich gathering phosphorus bacteria and its subgroup,activated sludge settleability in good condition.Temperature at 20 ?,25 ? and 30 ? contrast test results show that,with the increase of temperature: system on COD removal ability improved,effect on ammonia nitrogen removal is not big,adverse to the total phosphorus removal.In terms of sludge extracellular polymer,temperature results in the decrease of total EPS,reduced protein content,amylose content increased;In terms of OTU clustering analysis,temperature may lead to increase of microbial community diversity,system of Betaproteobacteria phosphorus mushroom group of relative abundance is reduced,the phylum Alphaproteobacteria glycan mushroom rise in relative abundance of the phylum Gammaproteobacteria glycan mushroom group of relative abundance changed little,the gaos strains for the advantages of microbial communities.Its change rule is consistent with the pollutants removal efficiency under different temperature.PH at 5.5,and 8.5 7 respectively the contrast test results show that,with the increase of pH,ammonia nitrogen and COD removal rate increases,the total phosphorus removal effects increase obviously;The total amount of the sludge EPS,protein and polysaccharide content is higher.Increases at the same time,the system of microbial community diversity,and its subgroup number increase,phosphorus accumulating gaos and its subgroup number changed little.Compared to peptone,glucose and acetate are good carbon source,the effluent is ideal,and the COD and ammonia nitrogen removal ability.Carbon source is glucose,the microbial community diversity,the largest amount of sludge EPS and polysaccharide content is the highest,mushroom group exhibited relative abundance;Acetate as carbon source,the system of optimal phosphorus removal capacity,relative abundance of mushroom group's largest phosphorus at this time.Dissolved oxygen concentration in 1 mg/L,3 mg/L and 5 mg/L of contrast test shows that the DO in the early period of the rise,the system of the removal rate of COD,TP and ammonia nitrogen are promoted,sludge settling sex good,DO rise in late,pollutant removal efficiency is no longer has obvious improvement;As the dissolved oxygen concentration increases,the total EPS reduce obviously,gaos and gradually reduce the relative abundance of the subgroup.With the increase of C/N,the dephosphorization efficiency of phosphorus accumulating gradually increased,thetotal sludge EPS,amylose content and protein content are reduced;Community diversity decreases,and the relative abundance of the GAOs exhibited increased,the relative abundance of the PAOs exhibited little change.Water C/N in 100/5,100/10 and 100/5 respectively the contrast test results show that,as the C/N,can lead to variation of phosphorus accumulating phosphorus removal,COD removal rate drop,sludge,the addition of protein,the greater the polysaccharide increase is less,and the higher the total EPS;With the increase of C/N,microbial community diversity increases,the decrease in the number of PAOs,chitosan GAOs number changed little.