Preparation Of CuFeSe₂ Nanomaterials And Its Application In Multimodality Imaging

Objective:A novel superparamagnetic nanomaterial CuFeSe₂was synthesized and then modified with a macromolecular copolymer methoxy polyethylene glycol-polycaprolactone?mPEG-PCL?to obtain a nanomaterial mPEG-PCL-CuFeSe₂,and then subjected to a series of Basic characterization,assessment of its in vitro and in vivo toxicity,and its feasibility in multimodality imaging.Methods:1.Preparation and characterization of CuFeSe₂ nanoparticles coated with macromolecule copolymer mPEG-PCL:?1?Firstly,CuFeSe₂ nanoparticles were synthesized.A simple aqueous phase method for synthesizing quantum dots was used to prepare selenium powder and sodium borohydride as precursors,and L-cysteine was used as a dispersion stabilizer to successfully prepare uniform size and size in pure water nitrogen?N2?protection environment.Monodisperse CuFeSe₂ nanoparticles.?2?The mPEG-PCL organics were prepared in the form of nanoparticles and mixed with CuFeSe₂ nanoparticles for ultrasonic dispersion for 4 hours.Then the nanomaterial mPEG-PCL-CuFeSe₂ was obtained and further centrifuged and purified.Its stability,characterization and magnetic properties were also studied.Evaluation of the Toxicity of mPEG-PCL-CuFeSe₂ Nanoparticles and Related Studies of Multimodality Imaging:?1?Different concentrations of mPEG-PCL-CuFeSe₂ nanoparticles and mouse breast cancer cells 4T1,human lung cancer cells A549,normal liver cells after incubating hepatocytes for 24hours,in vitro cytotoxicity evaluation was performed by thiazolyl blue colorimetry?MTT?.?2?The mPEG-PCL-CuFeSe₂ nanoparticles were injected through the tail vein of mice,and then the main organs of the mice were taken at different time points for section staining analysis to evaluate the toxicity in vivo.?3?Establishing a 4T1 subcutaneous xenograft tumor model of mouse breast cancer cells and injecting mPEG-PCL-CuFeSe₂ nanoparticles through the tail vein.Magnetic resonance imaging?MRI?and CT tomography were performed on the tumor region of the tumor mice before and after drug injection,and measure the magnetic resonance T₂ value,T₂ relative signal intensity,SIR tumor/muscle?T₂WI?value and CT values of the tumor area.Results:?1?Transmission Electron Microscopy?TEM?and High Resolution Transmission Electron Microscopy?HR-TEM?showed that CuFeSe₂nanoparticles were relatively uniform in size and the average particle size was?4.2±0.7?nm.X-ray single crystal diffractometer?XRD?results showed that the CuFeSe₂ and mPEG-PCL-CuFeSe₂ nanoparticles exhibit crystalline plane peaks at?112?and?220?,indicating that the nanoparticles are cubic crystal structures.Optical studies show that the experimentally synthesized Cu Fe Se₂,mPEG-PCL-CuFeSe₂ nanoparticles have absorption in the visible near-infrared region;Magnetic properties studies show that CuFeSe₂,mPEG-PCL-CuFeSe₂nanoparticles have good superparamagnetism.?2?In vitro cytotoxicity test results showed that CuFeSe₂ and mPEG-PCL-CuFeSe₂ nanoparticles had no obvious cytotoxicity in the above three cell lines at a concentration of 0-150?g/ml,and at the sameconcentration under the conditions,mPEG-PCL-CuFeSe₂ was less toxic to the three cell lines.?3?The results of long-term toxicity test in vivo showed that there were no obvious tissue necrosis and inflammation reactions in HE staining sections of the main organs?including liver,spleen,kidney,heart,lung?of the mice compared with the control group.?4?TSE T₂WI and M-GRASE sequence scans of MRI before and after the tail vein injection of mPEG-PCL-CuFeSe₂ nanoparticle solution showed that the T₂ value,T₂WI signal intensity values and SIR tumor/muscle?T₂WI?value of the tumor tissue in the experimental group were compared with those before the injection.The signal intensity values were all decreased,and the statistical results showed that there was a statistically significant difference in the T₂ values,T₂WI signal intensity values and SIR tumor/muscle?T₂WI?value between the mice before and after injection?P<0.05?.The results of CT scanning before and after direct intratumoral injection of mPEG-PCL-CuFeSe₂nanoparticle solution showed that the CT value of the tumor tissue in the experimental group was increased compared with that before the injection.The statistical results showed that there was a statistically significant difference in the CT values of the tumor tissue before and after injection?P<0.05?.Conclusion?1?We successfully prepared CuFeSe₂ nanoparticles and modified them with a high molecular weight polymer methoxy polyethylene glycol-poly caprolactone?mPEG-PCL?to obtain mPEG-PCL-CuFeSe₂ nanoparticles with good biocompatibility,and mPEG-PCL-CuFeSe₂ nanoparticles have good magnetic properties and CT imaging capabilities.The results of in vitro and in vivo toxicity studies showed that mPEG-PCL-CuFe Se₂ nanoparticles have good biological safety.?2?mPEG-PCL-CuFeSe₂ nanoparticles exhibited good imaging results in magnetic resonance T₂-weighted imaging and CT imaging.