Combined Remediation Of Oil-spilled Shoreline By Oil-degrading Enzyme And Biological Agents

Oil spill pollution in the sea has been more and more serious in the word,we can not ignore it any more.Since the 1990 s,people put forward the bioremediation method gradually.Biological treatment has been focused on because of its small side effect,not easy to cause secondary pollution.It restricted because of its strong dependence to the environment.Relative to microorganism,enzyme preparations has make up for this shortcoming,then apper remediation technology by bactria and enzyme.This experiment is mainly to optimize the environmental conditions of Pycnoporus coccineus HJZX DXL liquid fermentation and laccase production,make sure that it can output efficient laccase.Results show that: Pycnoporus coccineus HJZX DXL in cultured 118 h reach maximum biomass,the best condition is 30 ?,pH of 5,nutrients to glucose,aspartic acid,potassium dihydrogen phosphate and disodium hydrogen phosphate,inoculation amount to 1.5%.The best laccase activity is in pH 4.5,temperature 50?,at the same time,it has the best stability.We choose the C-1 as oil degradation bacteria its oil degradation rate was 25%.The oil degradation rate of enzyme with bacteria under the condition of pH 6,salinity 2%,enzymes and bacteria volume ratio 1:1 reached the maximum in 12 days.Because actual remediation is in open areas,many factors cannot be controlled,enzyme is easy to be diluted in water,can not reach the expected results.So the immobilized laccase were studied,results show that 5% glutaraldehyde concentration,crosslinking time 8h,under the condition of enzyme is 15 mg/g,it degrade oil with bacteria reached maximum oil degradation rate: 45%.Fixation enzyme in the condition pf pH 4.5,temperature 50 ?,enzyme activity reached the maximum.