The Preparation And Application Of Epitope Imprinting Polymers Based On Nitrogen-doped Graphene Quantum Dots

Molecular imprinting technique(MIT)is a technique that has a good specificity for the target molecule.The prepared molecular imprinting polymer(MIP)has good chemical stability and high selectivity.In the following decades,although MIP for small molecule synthesis is straightforward now,for protein imprinting is still a significant challenge due to the large molecular weights and the instability of the structure.The use of surface imprinting and epitope imprinting method overcomed the problems effectively.Nitrogen-doped graphene quantum dots(N-GQDs)have received increasing attention from the beginning due to their attractive properties,including large specific surface area,excellent photostability,stable fluorescence properties and eco-friendliness as so on.In this paper,the high selectivity of MIT and the high sensitivity of quantum dots were combined,and the fluorescent MIP was prepared with specific recognition for target by epitope imprinting.The details are as follows:Chapter 1: Introduction.The principle and classification of molecular imprinting technology and the three methods of molecular molecular imprinting for proteins were introduced.The properties and preparation methods of nitrogen-doped graphene quantum dots were briefly introduced.The related researchs of the combination of quantum dot and molecular imprinting technique were also introduced.Finally,the topic selection and innovation of this thesis were summarized.Chapter 2: The preparation of nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation.The receptor was prepared by surface imprinting procedure,epitope imprinting and metal chelation with silica-modified N-GQQDs as the carrier,the C-and N-terminal nonapeptides of cytochrome c as the common templates,zinc acrylate as functional monomer.The target protein was detected by fluorescence response and the detection of the target protein in the actual sample was realized.In this work,nitrogen-doped graphene quantum dots were applied to the preparation of epitope-based imprinted polymers,and the use of metal chelation and common templates increased the specificity of the polymer greatly.Chapter 3: The preparation of silica/nitrogen doped graphene quantum dots/epitope molecular imprinting polymer.The nitrogen-doped graphene quantum dots were linked onto the surface of amino-modified silica nanoparticles by covalent bonding and the composite particles played the role of fluorescence source and carrier.Then the polymer was synthesized with the C-terminal nonapeptides of transferrin as template and dopamine as the functional monomer.The imprinting layer was formed on the surface of carrier through the self-polymerization of dopamine in weak alkaline with enough air.The imprinting polymer has a certain specificity to transferrin.The operation of this work is easy,and reaction conditions is mild.This work provides a new material for further relevant research work.