| L-serine,which is anon-essential amino acidcan,could contribute positively to growth and development of human body,lipid metabolism as well as the immune system protecting us form disease,generally existing in the body of the spinal cord and cerebrospinal fluid.But pruduction and separation of L-serine were difficulty getting done for its rapid metabolism.Molecular imprinting technique is a efficient techniques to separate L-serine form mixed solution with the high-separate speeding.Materials Studio was used to simulate to screen reaction solvents form chloroform?THM?,acetonitrile?ACN?,dimethyl sulfoxide?DMSO?and functional monomers that was selected form methacrylic acid?MAA?,acrylamide?AA?,trifluoromethacrylic acid?TFMAA?.Molecularly imprinted polymers of L-serine was prearated based on magnetic carbon microspheres by living radical polymerization to separate L-serine according the simulation results.The results are shown below:?1?Magnetic carbon microspheres was synthesized by hydrothermal method with cheap and readily available glucose as a raw material,adding surfactants at the same time and using two types of iron salts?ferrous gluconate and ferric chloride?.A series of characterization methods such as scanning electron microscopy?SEM?and thermogravimetry?TG?were used to investigate the effect of reaction temperature and the type of surfactant on the morphology and surface functional groups of the products.The results show that:magnetic carbon microspheres were prepared by hydrothermal method at 180°C,reaction time 18 h,by adding cetyltrimethylammonium bromide?CTAB?(other conditions:C?glucose?=0.01 mol/L,C?ferric chloride?=0.002 mol/L,C?ferrous gluconate?=0.001 mol/L),the magnetic carbon microspheres have good sphericity and uniform distribution,and are good molecularly imprinted polymer matrices.?2?Optimization of L-serine molecularly imprinted polymers system:Doml3 module in materials studio of visualization software was used to optimize the structure of L-serine and the three functional monomers methacrylic acid?MAA?,acrylamide?AA?,trifluoromethacrylic acid?TFMAA?,then select a more suitable solvent system through comparing the minimum energy of L-serine in the chloroform?THM?,acetonitrile?ACN?,dimethyl sulfoxide?DMSO?.Next,a suitable molecular imprinted polymers system for L-serine was filtered out by comparing experimental data that by optimizating the system of L-serine-methacrylic acid?L-serine-MAA?,L-serine-acrylamide?L-serine-AA?,L-serine-trifluoromethacrylic acid?L-serine-TFMAA?in the selected solvent system.The results show that:L-serine molecularly imprinted polymers should be prepared by L-serine and methacrylic acid?MAA?in the ratio of 1:4 in the solvent dimethyl sulfoxide?DMSO?.?3?Preparation and adsorption properties of L-serine molecularly imprinted polymers:firstly,the anneale-magnetic carbon microspheres,modified by silylation of 3-chloropropyltrimethoxysilane,was reacted with sodium diethyldithiocarbamate?copper reagent?to obtain photocatalysed iniferter on magnetic carbon microspheres surface;then L-serine molecularly imprinted polymers on magnetic carbon microspheres was preparated according to the experimental design of simulation results,and its adsorption capacity and adsorption mechanism was studied.The results show that:L-serine molecularly imprinted polymer on magnetic carbon microspheres was prepared successfully.Adsorption model of L-serine molecularly imprinted polymers on magnetic carbon microspheres was in accordance with the Langmuir adsorption model,the maximum adsorption capacity was 12.7708 mg/g,and the kinetic adsorption fitting was in line with the pseudo-second-order kinetic models. |
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