Research On Changes Of Amino Acids And Microbial Diversity In Biological Carbon Sand Filter

With the quality of people's living standards continue to improve,drinking water safety issues are more and more people's attention.At present,the micro-pollution of water source has become the main problem that plagues China's water supply industry.Micro-polluted raw nitrogen in the water in the disinfection process will produce nitrogen disinfection by-products,to the drinking water safety to bring some risks.Therefore,it is important to study the production and control of dissolved organic nitrogen?DON?and related pollutants in water treatment.This paper mainly studies the biological sand filter,bio-charcoal filter and bio-carbon filter in drinking water process to generate DON generation regularity and removal mechanism based on amino acid,including the filter of different filter types in biofilter The effects of DON production on behalf of amino acids and the mechanism of control,as well as the structure of bacteria inside the biofilter,the results show that:1.Three kinds of biological filter After the membrane was stabilized,the average removal rates of NH₄⁺in biological sand filter and biological carbon sand filter were as high as 83.87%and 89.06%,respectively,which were better than that of biochar filter.Three kinds of biofilters The NO₂^-removal effect is obvious,the NO₂^-in the effluent is almost zero,and the mature DON results in the increase of DON due to the activity of microorganisms and the release of SMPs to water.2.Biofilter 11 kinds of amino acids in the mature membrane after the maturity of the filter to remove the effect of different cycles can be divided into the following categories:First,aspartic acid?Asp?in the filter to remove the obvious effect.Second,Thr,Ser,Glu,Gly and Cys increased first and then decreased and finally increased again.Third,Val,Ile,Leu,Tyr and Phe may not change obviously with the operation of the filter.It is due to its unique hydrophobic effect leading to these types of amino acids in the filter removal efficiency is low.3.DON in the filter along the trend of the trend was first decreased after the rise trend in 40-50cm at the lowest point,then rise.The effluent DON concentrations from high to low were biological sand filter,biological carbon filter and biological carbon sand filter.4.The surface properties of filter media affect the biomass attached to filter,thus affecting the changes of DON in the biofilter along the way.Comparing the characteristics of microbial colonies in three kinds of biological filter media samples,there are significant differences in bacterial flora structure on different filter media in biological filter.Among them,S3 and TS3 had the highest microbial abundance.In this study,the higher the microbial abundance in the filter,the better the degradation of amino acids.The species diversity of microorganisms in the filter media and the differences in the microbial population affected DON generation in the filter.A total of39 fungi were detected in 12 biofilters,with the highest abundance of Proteobacteria.Three biofilms,Elstera,Rhizorhabdus and Exiguobacterium,dominate the biofilter,all of which are associated with the degradation of amino acids.5.The comparison of functional microorganisms in 12 biofilm-adherent biofilms showed that Nitrosomonas was the dominant species in AOB bacteria and Nitrospira was the predominant bacteria in NOB bacteria The main dominant bacteria of denitrifying bacteria is Bacillu.