Study On The Application Of Iron Oxide Nanoparticles On Treating Iron Deficiency Chlorosis Of Plants

Iron is one of the important trace elements of organisms. Iron deficiency of plant will cause a serious problem called chlorosis. Most of the iron fertilizers can not solve this problem very well. Present work showed that some kinds of plants can uptake some kinds of nanoparticles and showed good results, including promoting seed germination, root elongation, photosynthesis and chlorophyll synthesis. Iron oxide nanoparticles have some special character, whether it can be absorbed by the plant has yet to be studied. The aim of this research is to prove the effect on the iron chlorosis correction and the related physiological of plant under iron deficiency stress.Seedlings of Citrus, cowpea, watermelon and corn were grown without iron in the nutrient solution. Then they were treated with different concentrations of Fe2O3 nanoparticles. Activities of SOD, POD and CAT, contents of soluble sugar, MDA, chlorophyll and soluble protein, activity of ferric reductase and root activity were tested to evaluate the growth of plant. We could explore the mechanism of plant biological effect under nanoparticles from the view of oxidative stress and the assimilation of the nanoparticles by the plant and its transport accumulation.The impact of nano-iron oxide on plant antioxidant enzyme activity research suggests that with an increase of concentration, activity of superoxide dismutase(SOD) did not have significant changes. With the increase of the concentration of nano-iron oxide, the activity of peroxidase(POD) was the lowest at the 20 mg/L of nano-iron oxide,while the catalase(CAT) activity reached the maximum when the concentration was 50mg/L, and the content of malondialdehyde(MDA) have been studied and, fluorescence microscope were also used to characterize in the root cells of plant, and we observed that the iron oxide nanoparticles were adsorpted onto the surface of plant root cells, and some also entered the plant root cells. This study could provide an important theoretical basis for the absorption and transportation of Fe2O3 nanoparticles, and the control of the iron chlorosis of plants.