| A 90-day growth experiment was conducted to investigate the dietary inositol requirement of juvenile Wuchang bream (Megalobrama amblycephala). The fish were randomly divided into six groups:a control group was fed with basal diet, and five treatment groups fed with basal diet supplemented with graded inositol levels (101.2, 202.3,404.8,809.1,1616.4 mg/kg of dry diet, respectively). Each diet (25 fish) was fed to triplicate groups of juvenile M. amblycephala with initial average body weight (3.4±0.07)g.1?Effects of dietary inositol on growth performance, physiological and biochemical indexes of serum and fat content in liver and muscle of juvenile Wuchang bream (Megalobrama amblycephala). At the end of feeding trial, final average weight (FAW), weight gain rate (WRG) and specific growth rate (SGR) of fish fed diet 404.8 mg/kg inositol were significantly higher than these of the control (P< 0.05); while feed conversion rate (FCR) was significantly lower in the group supplemented with 404.8 mg/kg inositol (P< 0.05). The concentrations of glucose (GLU), cholesterol (TC) and low density lipoprotein (LDL-C) were higher in fish fed 809.1mg/kg inositol (P< 0.05) and concentration of triglyceride (TG) was significantly lower (P< 0.05) than these of the control (P< 0.05); In addition, the fat content in liver and muscle of the groups supplemented with 404.8,809.1 mg/kg inositol were significantly lower than that of control (P< 0.05); wherase crude protein in muscle was significantly enhanced than that of control (P< 0.05). No significantly difference were observed in survival rate, hepatosomatic index (HSI), viscerosomatic ratio (VR), fullness coefficient (CF), high density lipoprotein (HDL-C) moisture and ash in muscle (P> 0.05). On the basis of SGR and FCR, the optimum dietary inositol requirement of juvenile M. amblycephala was estimated to be 294.44 mg/kg and 284.85 mg/kg, using broken-line regression analysis.2?Effects of dietary inositol on immune function of juvenile M. amblycephala under ammonia stress. After 90 days feeding, the results showed that, before ammonia stress, compared to the control group, Lym, C3, C4 and respiratory burst activities of fish fed diet 404.8 mg/kg inositol were significantly higher (P< 0.05); under ammonia stress for 12 hours, the WBC, RBC, Lym, HGB, C3 and C4 contents were significantly higher in the group supplemented with 404.8 and 809.1 mg/kg inositol groups (P< 0.05), respiratory burst activity was significantly enhanced than that of control (P< 0.05), cortisol level was significantly decreased than that of control (P< 0.05); under ammonia stress for 72 hours, the WBC, RBC, Lym, HGB, C3 and respiratory burst activities in fish fed diet containing 404.8 mg/kg inositol were significantly higher (P< 0.05), cortisol level was significantly decreased (P< 0.05), C4 levels was was significantly enhanced in the group supplemented with 809.1 mg/kg inositol compared to the values in the control group (P< 0.05). So, it is suggested that ingestion of a basal diet supplemented with inositol 404.8 mg/kg diet can enhance immune function and resistance against ammonia stress of juvenile M. amblycephala.3?Effects of dietary inositol on serum biochemical and antioxidant indexes of juvenile M. amblycephala under ammonia stress. After 90 days feeding, the results showed that before ammonia stress, compared to the control group, the serum GLU, LDL-C and CAT levels offish fed diet 404.8 mg/kg inositol were significantly higher (P< 0.05), the TG and MDA levels were significantly reduced (P< 0.05), the serum TC level was increased in 809.1 mg/kg group and the serum SOD level was reduced in 202.3 mg/kg group (P< 0.05); under ammonia stress for 12 hours, the GLU, LDL-C, CAT and SOD levels were significantly higher in the group supplemented with 404.8 mg/kg inositol groups (P< 0.05), the AST, TG and MDA levels were significantly decreased (P< 0.05), the serum TC levels of fish fed diet 404.8 mg/kg inositol were significantly higher (P< 0.05); under ammonia stress for 72 hours, the GLU, LDL-C, CAT and SOD levels fed diet 404.8 mg/kg inositol were significantly higher (P< 0.05), AST and MDA levels were significantly decreased (P< 0.05). So, it is suggested that ingestion of a basal diet supplemented with inositol 404.8 mg/kg diet can enhance the antioxidant ability of juvenile M. amblycephala.4?Effects of dietary inositol on genes expression of intestinal of juvenile M. amblycephala under ammonia stress. After 90 days feeding, the results showed that, before ammonia stress, compared to the control group, the expression levels of Mn-SOD, GSH-Px, CAT, Peroxiredoxin?, PeroxiredoxinVI, TNF-a and IL-lb offish fed diet 404.8 mg/kg inositol were significantly higher (P< 0.05), the expression levels of Peroxiredoxin V was significantly higher in the group supplemented with 809.1 and 1616.4 mg/kg inositol groups (P< 0.05); under ammonia stress for 12 hours, the expression levels of Mn-SOD, GSH-Px, CAT, Peroxiredoxin?, PeroxiredoxinVI, PeroxiredoxinVI, TNF-a and IL-lb was increased in 404.8 mg/kg group (P< 0.05); under ammonia stress for 72 hours, the expression levels of Mn-SOD, CAT, Peroxiredoxin?, PeroxiredoxinVI, Peroxiredoxin ?, PeroxiredoxinVI, TNF-a and IL-lb of fish fed diet 404.8 mg/kg inositol were significantly higher (P< 0.05). So, it is suggested that ingestion of a basal diet supplemented with inositol 404.8 mg/kg diet can enhance the expression levels of genes of intestinal of juvenile M. amblycephala under ammonia stress. |
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