Analysis Of The Difference Of Gene Expression S Between HB Red-Flower Line And It's Near-Isogeic Line In Cotton

HB red-flower trait was introdunced from wild diploid Gossypium bickii, and HB red-flower gene was primarily located on the chromosome 7. In this study, new SSR markers were employed to the fine mapping of HB gene. Gene microarray and proteomics technology were also applied to assess the HB near-isogenic lines for the related function analysis.1?SSR marker was used to the fine mapping the HB red-flower genes. According to result of the detection of the difference between near-isogenic lines, five markers,named A07-0646, A07-0592, A07-0594, CH07-44 and CH07-53, respectively, were selected to the construct a 81.8cM linkage map, and the HB gene was mapped between the A07-0592 and A07-0594, and the genetic distance respectively is 7.9cM and 37.6cM, respectively. According to the information of open genomic sequence,the physical distance between A07-0594 and A07-0592 was 33 Kb. A total of 21 genes were denoted in the genomic region, and 4 genes with the length more than 400 bp belongs to Methyltransferase MT-A70 family protein, glutathione S-transferase phi12, Class I glutamine amidotransferase-like superfamily protein,and PHYTOENE SYNTHASE, respectively. The results would provide the basis for the further cloning of important traits related genes and functional analysis and verification, which laidthe foundation for cotton development of new germplasm and genetic improvement.2?The difference of gene expression was analyzed with whole genomic microarray.A total of 80 genes were detected between HB red-flower near-isogenic lines,including 56 up-regulated genes and 24 down- regulated genes.These genes were mainly categorized as secondary and metabolism, biosynthesis, cell apoptosis, signal transduction related cDNAs and some unknown function genes. The expression of four genes selected by gene chip was analyzed with RT-PCR, and the results would provide useful information for clarifying the regulation of screening genes and factors of red-flower traits.3. The protein of different organs and developmental stages n of HB118 line and recurrent parent 118 line was analyzed with proteomics methods. A total of 157 proteins were detected with the expression level of more than 2 times difference,including 57 flowering leaf proteins, 56 boll leaf proteins, 44 flower proteins.Subsequently, 157 proteins were identified and functionally categorized as,(1)carbohydrate metabolism proteins;(2)photosynthesis proteins;(3)cytoskeleton related proteins;(4)cell defense proteins;(5)transport proteins;(6)transcriptional and translational regulatory proteins;(7)molecular partner proteins;(8)Hypothetical proteins;(9)other processes proteins. The structure and function of some proteinswas clarified, as well as the interaction networks and clusters regulation. The expression of10 different proteins selected by two-dimensional gel electrophoresis(2-DE) were validated with Q-PCR. These proteins were involved in the process of energymetabolism, photosynthesis, transcription and translation, etc. The results would lay the foundation for the further study of protein function.