QTL Analysis For Plant Height, Total Branching Number And Pod Traits In Peanut(Arachis Hypogaea L.)

In this study, a recombinant inbred line population of 151 lines, derived from a cross between variety 79266(P1) and its variant progeny D893(P2), was used to construct a peanut genetic map. and identify QTLs for 10 yield traits in five years(2011 to 2015) and two locations(Tai?an and Ji?ning).The main results were as follows:A genetic linkage map of cultivated peanut was constructed. The map consisted of 231 simple sequence repeat markers in 23 linkage groups, had a total length of 905.18 c M with average and minimum marker intervals of 3.92 and 0.1 c M.QTL identification for all 10 agronomic traits were identified by using ICIM model(Inclusive Composite Interval Mapping) in QTL Ici Mapping V4.0. A total of 96 QTLs were detected in seven environments. including eleven for main stem height, sixteen for lateral branch length, thirteen for total branching number, seven for pod length, six for pod width, nine for pod shell thickness, fourteen for single-pod weigh, t seven for single-seed weigh, six for shelling percentage, seven for total pod weight per-plant. every single QTL explained 5.66%~28.05% of the phenotypic variation explained(PVE).Eight QTLs were detected in three or more environments: one for main stem height, two for lateral branch length, one for total branching number, one for pod width, one for pod shell thickness, one for shelling percentage, and one for total pod weight per-plant; we also detected twenty-three area which associated with more than one trait. Six areas associated with three or more traits.The area ARS203~AHS1413 detected one lateral branch length QTL Qllb-11-1(which steadily detected in five environments), the corresponding DNA length of QTL marker region in the A. duranensis map was 44.7 kbp. The area Seq3D09~ARS535-1 detected one total branching number QTL Qtbn-17-1 w(which steadily detected in five environments), the corresponding DNA length of QTL marker region in the A. duranensis map was 92.6kbp. The area ARS376~Seq4G02 detected one main stem height QTL Qmsh-14-3(which steadily detected in five environments), the corresponding DNA length of QTL marker region in the genome physical map of A. duranensis was 6.87 Mbp.A QTL cluster area was detected between markers GM1901 and SEQ2G031 which associated with pod width, pod shell tickness, single-pod weight and pod shell tickness, the corresponding DNA length of QTL marker region in the A. duranensis map was 0.6Mbp. A QTL cluster area was detected between markers GM2289 and p PGPseq4E10 which associated with pod shell tickness, single-pod weight and pod shell tickness, the corresponding DNA length of QTL marker region in the A. duranensis map was 0.8Mbp. A QTL cluster area was detected between markers AHGS2244 and AHTE0839 which associated with pod width, total branching number, single-pod weight and single-seed weight, the corresponding DNA length of QTL marker region in the A. duranensis map was 2.2Mbp. A QTL cluster area was detected between markers AHGS1986 and seq19D09 which associated with lateral branch length and pod shell tickness, the corresponding DNA length of QTL marker region in the A. duranensis map was 2.4Mbp. A QTL cluster area was detected between markers ARS120 and GM1901 which associated with pod width, pod shell tickness and pod shell tickness, the corresponding DNA length of QTL marker region in the A. duranensis map was 4.5Mbp. A QTL cluster area was detected between markers AHGS2635 and AHTE0915 which associated with Main Stem Height,lateral branch length, Pod Shell Tickness and Total Pod Weight per-plant, the corresponding DNA length of QTL marker region in the A. duranensis map was 9.7Mbp.The QTL analysis result is useful to further understand the genetic basis of cultivated peanut, and provide reference for peanuts further QTLs identification, map-based cloning and molecular marker assisted breeding.