| Avian Leukosis Virus( ALV) was isolated for the first time since 1908 and reported since then decades all over the world with the outbreak of epidemic disease, suggesting that ALV has been widely distributed around the world, ALV caused clinical and subclinical infection make the poultry industry has brought great economic losses, but also attracted enough attention to the poultry industry. From the last century, the world’s major multinational companies have taken a series of measures to clear and control ALV.So in recent years, the developed countries of poultry industry has been basically brought under control and eradication of classical exogenous ALV in scale chicken farm, but in our country,ALV infection is still widespread. In pace with diagnostic methods became more and more,the attention of different diagnostic methods was getting higher and higher. So, choose a high sensitivity and specificity of the detection method not only can certainly make poultry farms and businesses effective control and clear avian leukosis, but also to reduce unnecessary losses and promote income.1.Different Ag-ELISA kits and colloidal gold test strip comparison with the detection of P27 in cell supernatantsTo evaluate different Ag-ELISA kit and colloidal gold test strip for avian leukosis virus,ALV-A, ALV-B, and ALV-J were inoculated into DF-1cells with five different doses(50,100, 500,1000,10000TCID50),changing cell maintenance fluid after 2h, the supernatants of these cells were harvested and tested for ALV group-specific antigen using different ELISA kits(A, B, C) and colloidal gold test strips(D, E) after the infected DF-1cells at 2-8 days. The results showed that the sensitivity was basically consistent with three different doses of subgroups of ALV by using ELISA kits(A, B, C), but the ELISA kit B was more sensitive than other two ELISA kits(A and C) in the individual dose of the three subgroups of ALV;the results were consistent with different colloidal gold test strips between D and E, however,the sensitivity was lower than ELISA kit and the detectable time were longer 1-2 days when inoculated little virus, but colloidal gold test strips could judged the result faster when inoculated numerous virus or in the late virus infection, that save more time than Ag-ELISA kits.2.Different Ag-ELISA kits and colloidal gold test strip comparison with the detection of P27 in eggs albumenIn this study, ALV-A, ALV-B, and ALV-J were inoculated into 90 SPF chickens using yolk sac inoculation. Collecting blood make virus isolation and detect antibody reaction every four weeks, Collecting eggs when they grown to the laying date. Chickens and eggs one to one, Total of 60 eggs albumen were collected and detected for P27 antigen of ALV using ELISA kits A, B and colloidal gold test strips D, E. Compared the result of the test and the result of virus isolation, The results showed that the match rate of colloidal gold strip and virus isolation does not exceed 60%, but the match rate of Ag-ELISA kits and virus isolation reaching 97.5%. So the positive rate for P27 antigen in eggs albumen using ELISA kits was higher than with colloidal gold test strips.So far, it cannot completely replace the ELISA kit in some applications. This study will be helpful for developing the detection method with high-sensitivity which is particularly useful in the breeding programs to eradication of avian leucosis virus infection. |
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