The Research Of Mhp On Processing And Presentation Function Of Porcine Pulmonary Alveolar Macrophages For Exogenous Antigen Effction

Pig asthma, also called swine enzootic pneumonia, swine enzootic pneumonia, more commonly referred to by scholars swine mycoplasma pneumonia (Mycoplasmal pneumonia of swine, MPS). It is a contagious animal diseases can contact. MPS is a pig caused by Mycoplasma pneumoniae (Mycoplasma hyopneumniae, Mhp) pathogen infection and disease due to being. In the research status at our and foreign country, the mortality rate of this disease is not high, but if other invasive pathogens cause secondary infection may cause a large number of pigs died of illness, Cause economic harm should not be underestimated, for farmers, hog Industry, an immense loss. The author is a comprehensive and accurate understanding of some areas of Hunan Province slaughtered pigs enzootic infections, as well as explore Mhp porcine alveolar macrophages (Porcine alveolar macrophages, PAM) presentation of exogenous antigen processing functions and what impact? The main results of this work and the resulting tests are as follows:On ordinary PCR method some large farms in Hunan Zhuzhou Liling, Yiyang Heshan, Liuyang Ge home xiangyin Yueyang, Shaoyang Sunning, Hengyang Leiyang six regions hyopneumoniae infection investigation, collecting pigs A total of 322 copies of the lung samples, using the boiling method to obtain crude sample DNA, respectively statistics in some areas of Hunan pig slaughterhouse enzootic infection rates. M. hyo found common ground in that the average infection rate was 20.8%(67/322), the infection rate details are as follows:Zhuzhou Liling 19.2%(11/57), Yiyang Heshan 18.1%(8/44), Liuyang GE hometown 20.0%(13/65), Yueyang xiangyin 17.5%(7/40), Shaoyang Sunning 23.2% (13/56), Hengyang Leiyang 25.0%(15/60). Positive products were sequenced organisms than the right, and has been published on the NCBI sequence homology as high as 95% to 99%. Pig slaughter by Mycoplasma pneumoniae infection among these six areas, a true understanding of the infection status of the land Mhp provide preliminary theoretical reference for future epidemiological investigations have focused on the incidence of regional intervention and prevention, thereby lowering infection proportion.Another method proposed be contagious in vitro, the PAM isolated and cultured, M. hyo support and co-incubation volume ratio by quantitative PCR to obtain the relevant factors and receptors related patterns nodes at different mutative times (12,24,36,48h). Toll-like receptor TLR2 measured by real-time quantitative PCR, TLR6, molecules CD86, and related genes:MHC Ⅱ, SLA-DM, Li chain mRNA expression nodes at different times the amount of change in the analysis of PAM exogenous infection Mhp Influence of antigen-presenting function. The effect point that:juxtapose with the control set, MHC Ⅱ molecule mRNA transcription level to peak at 12h post infection (83 times), could not have been detected at 48h; SLA-DM molecules transcribed mRNA reached the highest amount in 12 hours after infection (178 times) to 36 hours have not detected; Li molecular mRNA transcription level 12 after infection increased 17 fold to 48h has not detected; CD86 molecule mRNA transcription level 12 hours after the infection up to 181 times the control group,48 hours could not have been disclosed; TLR2 molecule mRNA transcription level 12h after infection that inhibited the expression level is 0.7 times the control group; 12h post infection TLR6 quickly increased to 380 times the control group, could not have been disclosed after 36h. Initially verified:Mhp to PAM can play for slaughter pigs produce immunosuppressive effect.The test confirmed the presence of Hunan parts of MPS fact provide the basis for the prevention and control of MPS, the farm will also provide the necessary theoretical and quarantine inspection reference. In addition, this trial also demonstrated that:Mhp for exogenous antigen-presenting function of processing PAM there is a strong inhibition. Lay the foundation for further research to find out who the pathogenesis of MPS, but also to strengthen the understanding of farm managers, operators of backyard farmers, farmers and other farmers on aspects of MPS disease, to a certain extent, is for them a useful and objective reference.