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Study Of The Process Improvements' Influence For Sea Cucumbers' Quality (Apostichopus Japonicus) In Industrial Culture

Posted on:2017-05-09Degree:MasterType:Thesis
Country:ChinaCandidate:Z Y ZhangFull Text:PDF
GTID:2323330488468234Subject:Food Science and Engineering
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In this paper,a new technology was used in the industrial aquaculture to solve the problem that the frequent changes of water had influenced on the sea cucumbers' quality,and we provided the analysis of nutritional components about the sea cucumbers from different sources for the lack of evaluation.During the experimental period,sucrose,activated sludge and EM was added to the aquaculture water.We used the National Standard Methods on physical and chemical properties of the water,studied changes of microbial community structure of the water with the help of high-throughput sequencing,and detected sea cucumbers' enzyme activity to analysis their quality changes.Experimental results showed that the method could maintain stable physical and chemical properties of the water.Carbon source demand for microbial growth was solved by adding sucrose,and raised chemical oxygen demand had no influence on sea cucumbers in an oxygen-rich experiment.The accumulation of hazardous substances such as nitrites was slower.By comparing the activity of alkaline phosphatase,acid phosphatase,lysozyme and superoxide dismutase in different sea cucumbers,the low concentration of ammonia nitrogen stress improved the enzyme activity,but the high stress inhibited the activity.During the experiment,the microbial community structure of the aquaculture water had obvious changes.Escherichia coli and pathogenic Vibrio were not detected.So the method was helpful to reduce the erosion of pathogens.We got the conclusion that the new technology could be used to improved the quality of sea cucumbers.Studying on the nutritional value and quality of sea cucumber in the industrial aquaculture,we compared the composition and nutritional quality of different sea cucumbers including samples in industrial culture,samples in pond culture,samples in bottom sowing culture and wild samples.The conclusion showed that the body wall production and the body wall production after cooking of samples in industrial culture was 62.7%~65.8% and24.8%~31.1%,at the same time,their average ASS amino acid score were 87.89~90.42.Comparing with other samples,they had higher amino acid nutrition level.But wild individuals grown in the natural environment,and they had a wide range of feeding and a longgrowth cycle.Therefor,wild individuals had higher level of protein and fat.We got the conclusion that sea cucumbers in industrial aquaculture had a high nutritional quality and actual production value.
Keywords/Search Tags:Apostichopus japonicus, process improvements, sucrose, EM, Bacterial Community, immunoenzyme, Nutritional analysis
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