Association Of Expression,Polymorphisms And Effect Of L-FABP And H-FABP Gene With Growth Traits In Guizhou White Goat

In order to study on the relationship of expression, polymorphisms and effect of L-FABP and H-FABP gene with growth traits in Guizhou White Goat, and be able to obtain molecular genetic marker for affecting growth traits of Guihzou White Goat. The technology of DNA pooling, direct sequencing and PCR-SSCP were used to discuss the single nucleotide polymorphism of L-FABP and H-FABP gene, and the model of GLM(SPSS 18.0) was used to correlation analysis different genotypes with growth traits index. Meanwhile, using online software to take the bioinformatics analysis of RNA structure and protein structures at all levels of L-FABP and H-FABP gene, and expression level of L-FABP and H-FABP gene in different gender and organizations of Guizhou White Goat was analyzed by Quantitative Real-time PCR. The major experimental results were as follows:1. The result of the polymorphism loci text showed that two SNPs(C4557T and A4575G) were tested in the Intron 2 of L-FABP gene in Guihzou White Goat and two sense mutation SNPs(G4713C and C8172T) were tested in the Exon3 and Exon5 of H-FABP gene in Guihzou White Goat.2. The result of the effect analysis showed that the polymorphic loci of C4557 T and A4575 G sites of L-FABP gene can be in favour of improve body weight, body height body length and pipe circumference with Guizhou White Goat. The genotype CCGG can be in favour of improve body height and body length in Guizhou White Goat adult ewes.The genotype TTGG can be in favour of improve body weight and pipe circumference inGuizhou White Goat aging ewes. The genotype CC of H-FABP gene C8228 T loci can be in favour of improve the total group of growth traits indicators with aging goats.3. The result of bioinformatics analysis showed that two mutations of H-FABP gene could cause changes in RNA secondary structure and protein structure had no influence at all levels. Both the physical and chemical properties of two proteins were stability and contained most of the amino acid residues were hydrophilic, and excluding the membrane structure domain and signal peptide, also did not belong to the membrane proteins or secreted protein, which was positioned in eukaryotes cytoplasm.4.The result of qRT-PCR text showed that L-FABP gene mRNA belonged to the liver and duodenum of nine groups of expression quantity was higher, then belonged to the heart and biceps femoris muscle expression quantity lowest in all adult Guizhou White Goat. H-FABP gene mRNA belonged to the heart and biceps femoris muscle of nine groups of expression quantity was higher, then belonged to the duodenum, lung and liver expression quantity lowest in adult Guizhou White Goat. There was an antagonism effect between two genes.