The Optimization Of Extraction For Cholorgenic Acid From Lepidium Latifolium L. And The Protpolast Fusion With Brassica Napus L.

Objectives Study the economic value of Lepidium latifolium L and the extraction technique of Chlorogenic acid from Lepidium latifolium L. Somatic hybridization technology was used to make Lepidium latifolium L. and Brassica napus L. conduct protoplast fusion and get the hybrid plants with fine properties, so as to achieve the improved varieties of Brassica napus L.Methods Through single factor experiment of ethanol concentration, material particle size and liquid-to-solid ratio and ultrasonic power of process parameters were studied,and through the response surface method to optimize the extraction process, a quadratic regression model was established. Using PEG- high Ca2+ and high p H induced fusion method to make the Lepidium latifolium L. mesophyll cell protoplast and Brassica napus L. mesophyll cell protoplast mix together dissymmetricly, and then in turn in solid-liquid Pell B double medium, Pell C amplification medium, Pell E rooting medium and MS medium culture.Develop a hybrid plant after carries on the morphology and cytology identification.Results Single factor and response surface optimization of chlorogenic acid extraction and the optimal parameters for: ethanol concentration 80%, 40 mesh material particle size,liquid-to-solid ratio 50:1, the ultrasonic power 200 w, under the experimental conditions the theoretical value of chlorogenic acid to obtain rate was 0.796%, the actual verification value is 0.712%. Through a series of experiments, the hybrid plants with fine properties was obtained.Conclusions Lepidium latifolium L. has very high economic value, this study confirmed that Lepidium latifolium L. can be used to extract chlorogenic acid; The protoplast hybridization technology was used to improve the Brassica napus L. The somatic hybridization between Brassica napus L and Lepidium latifolium L. will make Brassica napus L get better traits.