Influence Of Interferon Gamma On Microcirculation Of Auricle Related Factors By Proteomics Research

Disturbance of microcirculation is an important manifestation of the disease.Many medical reports have reported the cause of microcirculation disturbance in pika animal model. But there is no relevant reports on the formation of pig auricle microcirculation mechanism. Interferon-gamma as an important proinflammatory cytokines,plays an crucial role in the process of development in microcirculation disturbance. In this experiment,the piglets ear was regarded as our research object,using proteomic methods,discusses the effect of interferon-gamma on pig ear microcirculation related factors change.Methods:(1) IFN- gamma or normal saline were injected to piglets ear vein, the tip of ear tissue were collected, using i TRAQ proteomic methods to analysis the proteome differences;(2)According to the proteomics results,immunohistochemical method was used to determine the position of protein expression difference in the organization, and to verify the result of proteomics.Results:(1) 2444 proteins were identified in the ear tissue, 122 proteins which were significantly upregulated in the experimental group, 102 proteins were significantly decreased;(2) The main functions of the implementation of differential proteins between the two groups of samples in the body is platelet activation and regulation of deoxyribonclease activity and positive regulation of multicellular organismal process.(3)The significant enrichment of KEGG pathway in differential expression proteins is the interaction of plant pathogens, complement and coagulation cascade and NOD-like receptor signaling pathway.(4) Endothelial and inducible nitric oxide synthase were Located in blood and thecapillary network of hair follicles, nuclear factor kappa B's location in the hair follicles of the inner root sheath, outer root sheath; protein kinase C is positioned in the surrounding loose connective tissue; immunohistochemistry results and protein group study is consistent with the proteomics results.This study revealed the possible mechanism of the effect of IFN-gamma on porcine ear microcirculation, and provided theoretical basis for the follow-up study on the formation mechanism of porcine ear microcirculation disorder.