Effects Of Sethoxydim And Nacl Stress On Physiological Characteristics Of Foxtail Millet Callus

Field weed and and soil salinization have seriously influence on the yield and quality of Foxtail Millet. Applying plant tissue culture technology to cultivate new varieties can save time and labor, and has high rate of mutation. This experiment used inflorescence of Jin Gu 21 Foxtail Millet as explants and do further optimization on the induction and regeneration conditions of inflorescence-derived callus. And respectively study on the effects of different concentration of Sethoxydim and Nacl on the induction, Growth, differentiation and the changes of physiological indexes of inflorescence-derived callus. In order to provide theoretical basis for screening herbicide resistant and salt tolerant mutant and genetic transformation of Foxtail Millet. The results show that:1. The optimal induction Medium of inflorescence-derived callus was MS+2.25mg/L2,4-D+ 0.5mg/LKT, the optimum subculture medium was MS+6-BA2.25mg/L+NAA0.5mg/L, and the best differentiation medium was MS+1.125mg/L6-BA+1.5% sucrose+0.25mg/LNAA.2. Sethoxydim and Nacl had marked inhibitory effect on the induction, Growth, differentiation of Foxtail Millet inflorescence-derived callus. No callus was obtained in the indution medium that added Sethoxydim and Nacl; The relative growth of callus decreased with the concentration of the Sethoxydim and Nacl increased. The inhibitory effect on the differentiation of callus also gradually increased with sethoxydim and Nacl concentration increased.3. With the extension of stress time, the SOD activity, POD activity and soluble protein content of callus increased at first and then decreased under different concentrations of Sethoxydim; The proline content in the callus was always at a high level when the concentrations of Sethoxydim was less than 1 ml·L-1, while decreased gradually when the concentration of Sethoxydim was greater than 1 ml·L-1. The MDA content accumulated slowly when the concentrations of Sethoxydim was less than 1 ml·L-1, whlie increased rapidly in the late stage of stress (16-32h) when the concentration of Sethoxydim was greater than 1 ml·L-1. The results show that, the callus of Jin Gu 21 Foxtail Millet has an adaptability to Sethoxydim environment, but its adaptability is low (≤1 mL·L-1 Sethoxydim).4. With the extension of stress time, the SOD activity, soluble protein content and proline content in callus increased at first and then decreased under different concentrations of Nacl; The POD activity in the callus rised continutly when the concentrations of Nacl was less than 0.5%, while increased at first and then decreased when the concentration of Nacl was greater than 0.5%. The MDA content accumulated slowly when the concentrations of Nacl was less than 0.5%, whlie increased rapidly in the late stage of stress (32h) when the concentration of Nacl was greater than 0.5%. The results show that, the callus of Jin Gu 21 Foxtail Millet has an adaptability to Nacl environment, but its adaptability is low (≤0.5% Nacl).