| Lipasin (also named Angptl8, betatrophin, C19orf80 and RIFL), is a new member of angiopoietin-like protein family. As a nutritional regulator, it is mainly present in the liver. The present study was conducted to explore whether oxidant stress affect the expression of porcine Lipasin gene. As a new functional amino acids, the present study investigated whether glutamate could reduce the expression of Lipasin by inhibiting oxidative stressIn the present study, Landrace × Yorkshire pigs were uesed as experimental animals. Using PCR, T-A cloning, RACE, and other experimental techniques to clone Lipasin the full length of cDNA gene.The present result showed that the full-length of this gene was 875 bp. Its ORF region contained 597 bp, from 21bp to 618bp, had high conservation. The protein of Lipasin had 198 amino acids. Its relative molecular mass was 21,984.3 Dalton. Its theoretical isoelectric point (pI) was 6.86.According the full-length sequence of porcine Lipasin gene, RT-PCR primers of Lipasin were designed. Housekeeping gene RPL32 was used as internal marker gene. Fluorescence quantitative was used to analysis the mRNA expression of porcine Lipasin gene in seven tissues including heart, liver, spleen, lung, kidney, back longest muscle, and abdominal fat. The results showed that Lipasin in healthy pigs was mainly expressed in the liver. Its expression in heart and adipose tissue was minimum.To analyse the effect of glutamate on the expression of Lipasin gene under oxidative stress,15 pigs were randomly divided into 3 group (N= 5):control group, hydrogen peroxide group, glutamate group. Present results demonstrated that Lipasin gene expressions in heart, liver, spleen, fat of hydrogen peroxide group were higher than in that the control group (P=0.01). Its expression in the other tissue was not significantly different. Glutamate could significantly inhibit the increased expression of Lipasin gene in these tissues including heart, liver, spleen, and fat (P=0.01). |
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