On The Micro Flora Carried By The Haemaphysalis Flava Tick |
Posted on:2017-08-19 | Degree:Master | Type:Thesis |
Country:China | Candidate:A D Zou | Full Text:PDF |
GTID:2323330512468520 | Subject:Veterinary Medicine |
Abstract/Summary: | PDF Full Text Request |
In this paper, we mainly by the technique of PCR-DGGE and high-throughput sequencing technology to analyzes the micro flora from the Haemaphysalis flava tick which are in different gender and stages.Results:1.Based on analysis of DGGE; Haemaphysalis flava tick intestinal contents; Article 11 the obvious stripe sequence respectively with Rickettsia peacockii?Rickettsia helvetica?Rickettsia slovaca?Rickettsia tarasevichiae?Klebsiella pneumoniae Staphylococcus?Pectobacterium carotovorum?Pseudomonas aeruginosa?Erwinia?Bacillus cereus and cultivate the soil bacterial 16 s rDNA V3 area sequence is highly similar.Results:2. Based on analysis of High-throughput sequencing technology; The high relative abundance of egg are Mycobacterium?Balneimonas and Phenylobacterium; The high relative abundance of larva are Methylosinus and Corynebacterium; The high relative abundance of nymph are Luteibacter?Propionibacterium?Coxiella?Serratia?Shewanella? Pseudomonas?Enhydrobacter and Staphylococcus?The high relative abundance of H.flava are Sphingomonas?Rickettsia?Brevibacterium and Ehrlichia. |
Keywords/Search Tags: | Haemaphysalis flava, DGGE, High-throughput sequencing |
PDF Full Text Request |
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