Features And Correlation Of Phosphorus And Microbial Community In Warming-induced Wetland Soils

Wetlands play unique ecological roles in regulating climate,water conservation and global biodiversity storage.Global warming has exerted extensive impacts on worldwide wetlands,including the influence on biogenic element cycling.Phosphorus has been regarded as a key priming factor causing water eutrophication,and biogeochemical cycling of phosphorus in wetland soils has close relationship with water eutrophication.It is known that microbe play crucial roles in biogeochemical cycling of phosphorus in wetland soils,however,the influence of global warming on microbe in wetland soils is also uncertain.In this study,a warming-induced wetland microcosm platform was set up and six wetland regions with different phosphorus characteristic were selected as the objects of study around the south Region of Yangtze River Delta.Which were performed to explore the relationship and dynamic response of phosphorus and microbial community in wetland soils under warmed condition.In addition,31P nuclear magnetic resonance(31P-NMR)technology and high-throughput sequencing of 16S rRNA gene were used to characterize P forms and identify microbial community composition respectively.And the relationship was demonstrated between microbial community composition and phosphorus forms under warmed condition from the perspective of statistic.The main results obtained in this study were as follows:(1)At seasonal and round-year scales,these species with significant difference under warmed condition were mainly from Acidobacteria,Actinobacteria,Proteobacteria,Firmicutes,Bacteroidetes,Nitrospirae,Chloroflexi,Planctomycetes and so on.In spring,under warming-induced condition,relative abundance of Acidobacteria decreased significantly up to 11%under low soil nutrition state,while relative abundance of Nitrospirae increased significantly up to 43%.In summer,under warming-induced condition,relative abundance of Bacteroidetes decreased up to 33%and relative abundance of Chloroflexi increased up to 7%significantly under moderate soil nutrition condition.In fall,under warming-induced condition,relative abundance of Firmicutes decreased up to 80%,74%and 72%significantly under low,moderate and high soil nutrition states respectively.Relative abundances of Acidobacteria and Chloroflexi increased significantly up to 7%and 42%respectively under low soil nutrition state,Relative abundances of Proteobacteria,Acidobacteria,Chloroflexi increased significantly up to 18%,24%and 12%under moderate soil nutrition state respectively.Relative abundance of Proteobacteria increased significantly up to 43%.In winter,under warming-induced condition,relative abundance of Nitrospirae increased significantly up to 54%and 66%under low and moderate soil nutrition states respectively.At round-year scale,under warming-induced condition,relative abundance of Firmicutes decreased up to 63%,50%and 56%significantly under low,moderate and high soil nutrition states respectively.Relative abundances of Chloroflexi and Planctomycetes increased significantly up to 30%and 13%respectively.Relative abundance of Bacteroidetes decreased significantly up to 33%under moderate soil nutrition state,while Proteobacteria and Nitrospirae increased significantly up to 56%and 8%respectively.Relative abundances of Proteobacteria and Planctomycetes increased significantly up to 29%and 13%under high soil nutrition state respectively.(2)Soil phosphorus(P)forms were dominated by orthophosphate and phosphomonoester under low,moderate and high nutrition states with different response at seasonal and round-year scales.In spring,under warming-induced condition,relative abundance of orthophosphate decreased significantly up to 33%,while phosphodiester increased significantly up to 113%under high soil nutrition state.In summer,under warming-induced condition,relative abundances of pyrophosphate and polyphosphate decreased significantly up to 38%and 40%under low soil nutrition state respectively.In winter,under warming-induced condition,relative abundance of phosphonate increased significantly up to 136%under high soil nutrition state.Notably,P forms had no significant difference in fall.At round-year scale,under warming-induced condition,relative abundances of phosphodiester and pyrophosphate increased significantly up to 60%and 86%under high soil nutrition state respectively.relative abundances of phosphomonoester,phosphonate,pyrophosphate and polyphosphate increased significantly up to 13%,46%,37%and 23%under moderate soil nutrition state respectively.And relative abundances of orthophosphate decreased significantly up to 10%and 16%under moderate and high soil nutrition states respectively.(3)Under warming-induced condition,obvious increase of Proteobacteria had significantly negative correlation with decrease of orthophosphate under under moderate and high soil nutrition states,which had significantly positive correlation with phosphodiester under high soil nutrition state and phosphomonoester under moderate soil nutrition state.(4)The research revealed warming had significant effects on microbial community and phosphorus forms in wetland soils,as well as significant changes of microbial community can trigger obvious changes of phosphorus forms under warming-induced condition,which provides new insights into understanding biogeochemical cycling of phosphorus in wetland soils under global climate warming condition.