The Genetic Diversity Analysis Of Rhodiola Sp.and Its Endophytic Fungi By ISSR And RAPD Molecular Markers

Rhodiola L.mainly distributed in the southwest,northeast,northwest and north China and other alpine mountains in China.Pharmacological studies show that Rhodiola has enhanced exercise tolerance,anti-oxidation,anti-diabetes,physical activity,is widely used in medicine,health care products,cosmetics and other industries.In this study,RAPD and ISSR molecular markers were selected to analyze the genetic diversities of four kinds of wild Rhodiola sp.to better protect and develop Rhodiola resources;On the other hand through its antioxidant activity of strains and host plant co-cultivation,raw correlation between host and fungal gene clusters within the study;Finally endophytic fungi from 23 genetic diversity to investigate whether clustering fungal sources related to its host.To study the molecular genetic diversity of endophytic fungi of Rhodiola L.and its and the correlation between them.The results showed that:(1)A total of 96 bands were amplified by 11 RAPD primers,and the percentage of polymorphism was 90.62%;102 bands were amplified by 11 ISSR primers,and the percentage of polymorphism was 100%.So the polymorphism detection ability of ISSR marker is higher than that of RAPD marker;Clustering analysis indicated that the samples were clustered into three categories by ISSR,RAPD + ISSR,and four categories by RAPD.Closer genetic distance between R.angusta and R.sachalinensis,R.crenulata and R.bupleuroides.Both ISSR and RAPD markers were efficient methods at revealing in interspecific or intraspecific genetic differences and diversity of plants in Rhodiola L..(2)The genetic diversity of host plant was studied by inoculating endophytic fungi ZPRs-R-11 which interaction 15 days,taken once a day sample.A total of 87 bands were amplified by 12 RAPD primers,and the percentage of polymorphism was83.91%;The vaccine described interactions of fungi and host plant have a lower Gene flow(Nm = 0.2098<1)shows that genetic exchange occurs.Clustering analysis indicated that the samples were clustered into three categories by RAPD markers when while the genetic similarity coefficient of 0.7400.The genetic diversity of host plant was studied by inoculating endophytic fungi ZPRa-R-1 which interaction 15 days,taken once a day sample.A total of 94 bands were amplified by 12 IS SR primers,and the percentage of polymorphism was87.23%;The vaccine described interactions of fungi and host plant have a lower Gene flow(Nm = 0.2098<1)shows that genetic exchange occurs.Clustering analysis indicated that the samples were clustered into three categories by ISSR markers when while the genetic similarity coefficient of 0.7400 which as the same as RAPD.(3)The genetic diversity of 23 endophytic fungi were analyzed by ISSR molecular marker.A total of 142 bands were amplified by 11 ISSR primers,and the percentage of polymorphism was 99.29%;The endophytic fungi which isolated from R.sachalinensis have a higher genetic diversity.Clustering analysis indicated that the samples were clustered into four categories by ISSR markers when while the genetic similarity coefficient of 0.7200.The genetic similarity coefficient of the same genus between high genetic similarity between different species and for low genetic similarity between the host-derived strains no significant correlation.